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Mouse Anti-Human TRAIL ELISA Set

PRINCIPLE OF ASSAY
This assay incorporates a quantitative sandwich enzyme immunoassay technique. Unlabeled monoclonal antibody specific for human TRAIL is first coated onto 96 well microplate(s). Standards and samples are then added to the wells and any TRAIL present is captured by the immobilized antibody. After a wash to remove unbound material, biotin-labeled anti-human TRAIL detection antibody is added to the wells, followed by a horseradish peroxidase-labeled anti-biotin antibody incubation. After another wash, TMB substrate solution is added that will result in a blue color proportional to the amount of bound TRAIL. Color development is then quenched and intensity is measured at 450nm.
[Technical Bulletin / References ] Back to Cytokine Research - Human
 Cat. No.Product DescriptionCloneSize
USD Price
Order Qty
11045-01
 
Anti-Human TRAIL ELISA Set
Set
$450.00
FORMAT ABBREVIATIONS:
UNLB - purified, unlabeled antibody
FITC - fluorescein isothiocyanate-labeled
TRITC - tetramethlyrhodamine isothiocyanate
AP - alkaline phosphatase-labeled
HRP - horseradish peroxidase-labeled
BGAL - B-galactosidase-labeled
TXRD - Texas RedTM
BIOT - biotin-labeled
R-PE - R-phycoerythrin-labeled
R-PE/TXRD - R-phycoerythrin/Texas RedTM
APC - allophycocyanin
 CY3 - CyanineTM 3-labeled *
SPRD - SpectralRedTM-labeled *
LE/AF - low endotoxin/azide-free
CY5 - CyanineTM5-labeled *
R-PE/CY5.5 - R-phycoerythrin/CyanineTM5.5-labeled *
R-PE/CY7 - R-phycoerythrin/CyanineTM7-labeled *
APC/CY5.5 - allophycocyanin/CyanineTM5.5-labeled *
APC/CY7 - allophycocyanin/CyanineTM7-labeled *
FLMA - fluorescein-5-malemide-labeled
SEPH - sepharose-labeled
BIMA - biotin-maleimide-labeled
NOTE: *SpectralRedTM (SPRD) is a tandem conjugate of R-phycoerythrin and CYTM5. It was developed as a reagent for 3-color flow cytometry on instruments with only 488 nm excitation source. It requires minimal (0-2%) FL2/FL3 compensation.