Keyword or Cat. No.

Mouse anti-Human Interleukin-21 (IL-21)-ELISA Set

DESCRIPTION

Interleukin-21 (IL-21) is a four helical bundle type I cytokine[1]. The mature form of human IL-21 is a 131 amino acid protein.[4] IL-21 is expressed primarily by NKT and CD4+ T cells, particularly the inflammatory Th17 subset and has pleiotropic effects on both innate and adaptive immune responses. The effects of IL-21 include promotion of antibody production, NK cell differentiation, proliferation and differentiation of cells of monocyte and granulocyte lineages, costimulation of T cells and augmentation of antitumor activity of CD8+ T cells.[2,3] Recent studies have shown that IL-21 is also an autocrine cytokine that potently induces Th17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases. Principle of Assay This assay incorporates a quantitative sandwich enzyme immunoassay technique. Unlabeled monoclonal antibody specific for human IL-21 is first coated onto 96 well microplate(s). Standards and samples are then added to the wells and any IL-21 present is captured by the immobilized antibody. After a wash to remove unbound material, biotin-labeled anti-human IL-21 detection antibody is added to the wells, followed by a horseradish peroxidase-labeled anti-biotin antibody incubation. After another wash, TMB substrate solution is added that will result in a blue color proportional to the amount of bound IL-21. Color development is then quenched and intensity is measured at 450nm.

[Technical Bulletin / References ]

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Cat.no.

Product Description

Format

Size

USD Price

Order Qty

15920-01		Anti-Human IL-21 ELISA Set			$450.00
			UNLB	Set


	FORMAT ABBREVIATIONS:

	UNLB - purified, unlabeled antibody FITC - fluorescein isothiocyanate-labeled TRITC - tetramethlyrhodamine isothiocyanate AP - alkaline phosphatase-labeled HRP - horseradish peroxidase-labeled BGAL - B-galactosidase-labeled TXRD - Texas Red^TM BIOT - biotin-labeled R-PE - R-phycoerythrin-labeled R-PE/TXRD - R-phycoerythrin/Texas Red^TM APC - allophycocyanin CY3 - Cyanine^TM 3-labeled *		SPRD - SpectralRed^TM-labeled * LE/AF - low endotoxin/azide-free CY5 - Cyanine^TM5-labeled * R-PE/CY5.5 - R-phycoerythrin/Cyanine^TM5.5-labeled * R-PE/CY7 - R-phycoerythrin/Cyanine^TM7-labeled * APC/CY5.5 - allophycocyanin/Cyanine^TM5.5-labeled * APC/CY7 - allophycocyanin/Cyanine^TM7-labeled * FLMA - fluorescein-5-malemide-labeled SEPH - sepharose-labeled BIMA - biotin-maleimide-labeled

	NOTE: *SpectralRed^TM (SPRD) is a tandem conjugate of R-phycoerythrin and CY^TM5. It was developed as a reagent for 3-color flow cytometry on instruments with only 488 nm excitation source. It requires minimal (0-2%) FL2/FL3 compensation.


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