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Rat Anti-Mouse CD25 (IL-2Rα)

ANTIGEN DESCRIPTION
The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity α chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.[14] CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.[2,3,15] Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated.[4] CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD.[5] It is expressed at a higher level on CD4+CD8+ thymocytes.[5,6] It is also expressed on cultured epidermal Langerhans cells.[7] The biochemical features of murine CD25 have been characterized in detail.8-10 The 3C7 monoclonal antibody reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4.[2,11,12] 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone .[2,13]
[Technical Bulletin / References ] Back to CD Markers and Other Cell Surface Proteins - Specificity Mouse
 Cat. No.Product DescriptionCloneSize
USD Price
Order Qty
1600-01
 
Rat Anti-Mouse CD25/IL-2Ra-UNLB
3C7 0.5 mg
$192.00
1600-02
 
Rat Anti-Mouse CD25/IL-2Ra-FITC
3C7 0.5 mg
$212.00
1600-08
 
Rat Anti-Mouse CD25/IL-2Ra-BIOT
3C7 0.5 mg
$208.00
1600-09
 
Rat Anti-Mouse CD25/IL-2Ra-R-PE
3C7 0.1 mg
$178.00
1600-09L
 
Rat Anti-Mouse CD25/IL-2Ra-R-PE
3C7 0.2 mg
$245.00
1600-11
 
Rat Anti-Mouse CD25/IL-2Ra-APC
3C7 0.1 mg
$245.00
1600-13
 
Rat Anti-Mouse CD25/IL-2Ra-SPRD
3C7 0.1 mg
$225.00
1600-14
 
Rat Anti-Mouse CD25/IL-2Ra-LE/AF
3C7 0.5 mg
$255.00
1600-17
 
Rat Anti-Mouse CD25-IL-2Ra-PE/CY7
3C7 0.1 mg
$192.00
FORMAT ABBREVIATIONS:
UNLB - purified, unlabeled antibody
FITC - fluorescein isothiocyanate-labeled
TRITC - tetramethlyrhodamine isothiocyanate
AP - alkaline phosphatase-labeled
HRP - horseradish peroxidase-labeled
BGAL - B-galactosidase-labeled
TXRD - Texas RedTM
BIOT - biotin-labeled
R-PE - R-phycoerythrin-labeled
R-PE/TXRD - R-phycoerythrin/Texas RedTM
APC - allophycocyanin
 CY3 - CyanineTM 3-labeled *
SPRD - SpectralRedTM-labeled *
LE/AF - low endotoxin/azide-free
CY5 - CyanineTM5-labeled *
R-PE/CY5.5 - R-phycoerythrin/CyanineTM5.5-labeled *
R-PE/CY7 - R-phycoerythrin/CyanineTM7-labeled *
APC/CY5.5 - allophycocyanin/CyanineTM5.5-labeled *
APC/CY7 - allophycocyanin/CyanineTM7-labeled *
FLMA - fluorescein-5-malemide-labeled
SEPH - sepharose-labeled
BIMA - biotin-maleimide-labeled
NOTE: *SpectralRedTM (SPRD) is a tandem conjugate of R-phycoerythrin and CYTM5. It was developed as a reagent for 3-color flow cytometry on instruments with only 488 nm excitation source. It requires minimal (0-2%) FL2/FL3 compensation.