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Mouse Anti-Mouse H-2Db

ANTIGEN DESCRIPTION
The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic α chains noncovalently associated with the invariant β2-microglobulin.[3,4] These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.[5] MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.[3,6] The monoclonal antibody 28-14-8 binds to the α3 domain of H-2Db in the presence or absence of β2 microglobulin.[7,8] It cross reacts with the α3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes,9-11 but it does not block recognition of H-2Ld-positive target cells by Ly- 6G2-positive NK cells.[12]
[Technical Bulletin / References ] Back to CD Markers and Other Cell Surface Proteins - Specificity Mouse
 Cat. No.Product DescriptionCloneSize
USD Price
Order Qty
1910-01
 
Mouse Anti-Mouse H-2Db-UNLB
28-14-8 0.5 mg
$208.00
1910-02
 
Mouse Anti-Mouse H-2Db-FITC
28-14-8 0.5 mg
$265.00
1910-08
 
Mouse Anti-Mouse H-2Db-BIOT
28-14-8 0.5 mg
$225.00
1910-09
 
Mouse Anti-Mouse H-2Db-R-PE
28-14-8 0.1 mg
$185.00
1910-14
 
Mouse Anti-Mouse H-2Db-LE/AF
28-14-8 0.5 mg
$315.00
FORMAT ABBREVIATIONS:
UNLB - purified, unlabeled antibody
FITC - fluorescein isothiocyanate-labeled
TRITC - tetramethlyrhodamine isothiocyanate
AP - alkaline phosphatase-labeled
HRP - horseradish peroxidase-labeled
BGAL - B-galactosidase-labeled
TXRD - Texas RedTM
BIOT - biotin-labeled
R-PE - R-phycoerythrin-labeled
R-PE/TXRD - R-phycoerythrin/Texas RedTM
APC - allophycocyanin
 CY3 - CyanineTM 3-labeled *
SPRD - SpectralRedTM-labeled *
LE/AF - low endotoxin/azide-free
CY5 - CyanineTM5-labeled *
R-PE/CY5.5 - R-phycoerythrin/CyanineTM5.5-labeled *
R-PE/CY7 - R-phycoerythrin/CyanineTM7-labeled *
APC/CY5.5 - allophycocyanin/CyanineTM5.5-labeled *
APC/CY7 - allophycocyanin/CyanineTM7-labeled *
FLMA - fluorescein-5-malemide-labeled
SEPH - sepharose-labeled
BIMA - biotin-maleimide-labeled
NOTE: *SpectralRedTM (SPRD) is a tandem conjugate of R-phycoerythrin and CYTM5. It was developed as a reagent for 3-color flow cytometry on instruments with only 488 nm excitation source. It requires minimal (0-2%) FL2/FL3 compensation.