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Mouse Anti-Chicken TCR αβ (Vβ2)

ANTIGEN DESCRIPTION
MAb TCR3 precipitates a CD3-associated heterodimer of Mr 88-kDa (two bands of Mr 48-kDa and 40- kDa upon reduction) on chicken peripheral blood T cells.[1,2] Deglycosylation of the heterodimer yields two polypeptides of Mr 34-kDa and 31-kDa from TCR3 precipitates. In the chicken, two distinct subpopulations of αβ T cells appear in the thymus subsequent to the appearance of γδ T cells. These subpopulations, originally denoted as TCR2 and TCR3, [1,2] arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vβ families, Vβ1 and Vβ2, respectively.[3] The TCR3 MAb reacts with approximately 9% of thymocytes, 15-25% of blood mononuclear cells and 13% of splenocytes young adult chickens. Two-color immunofluorescence has revealed that the TCR3+ thymocytes include CD4+ CD8- , CD4- CD8+ , CD4+ CD8+ and CD4- CD8- subpopulations. The TCR3+ thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR3/CD3 complex and most of the cells in this subset are CD4+ CD8+ . Cells in the other subset express TCR3/CD3 in higher density and are either CD4+ CD8- or CD4- CD8+ , corresponding to the more mature medullary subset of thymocytes. The TCR3+ cells in the blood and spleen express relatively high levels of the TCR3/CD3 receptor complex and are “single positive, with CD4+ CD8- cells being four times more frequent that the CD4- CD8+ cells (ca.80 0% CD4+ vs ca.20 0% CD8+ ).[1,2]
[Technical Bulletin / References ] Back to CD Markers and Other Cell Surface Proteins - Specificity Chicken
 Cat. No.Product DescriptionCloneSize
USD Price
Order Qty
8250-01
 
Mouse Anti-Chicken TCRab/Vb2-UNLB
TCR-3 0.5 mg
$240.00
8250-02
 
Mouse Anti-Chicken TCRab/Vb2-FITC
TCR-3 0.5 mg
$340.00
8250-08
 
Mouse Anti-Chicken TCRab/Vb2-BIOT
TCR-3 0.5 mg
$305.00
8250-09
 
Mouse Anti-Chicken TCRab/Vb2-R-PE
TCR-3 0.1 mg
$215.00
8250-13
 
Mouse Anti-Chicken TCRab/Vb2-SPRD
TCR-3 0.1 mg
$260.00
FORMAT ABBREVIATIONS:
UNLB - purified, unlabeled antibody
FITC - fluorescein isothiocyanate-labeled
TRITC - tetramethlyrhodamine isothiocyanate
AP - alkaline phosphatase-labeled
HRP - horseradish peroxidase-labeled
BGAL - B-galactosidase-labeled
TXRD - Texas RedTM
BIOT - biotin-labeled
R-PE - R-phycoerythrin-labeled
R-PE/TXRD - R-phycoerythrin/Texas RedTM
APC - allophycocyanin
 CY3 - CyanineTM 3-labeled *
SPRD - SpectralRedTM-labeled *
LE/AF - low endotoxin/azide-free
CY5 - CyanineTM5-labeled *
R-PE/CY5.5 - R-phycoerythrin/CyanineTM5.5-labeled *
R-PE/CY7 - R-phycoerythrin/CyanineTM7-labeled *
APC/CY5.5 - allophycocyanin/CyanineTM5.5-labeled *
APC/CY7 - allophycocyanin/CyanineTM7-labeled *
FLMA - fluorescein-5-malemide-labeled
SEPH - sepharose-labeled
BIMA - biotin-maleimide-labeled
NOTE: *SpectralRedTM (SPRD) is a tandem conjugate of R-phycoerythrin and CYTM5. It was developed as a reagent for 3-color flow cytometry on instruments with only 488 nm excitation source. It requires minimal (0-2%) FL2/FL3 compensation.