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IMMUNOCHEMISTRY SERVICES
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FRAGMENTATION SERVICES
Antibody fragmentation services for 5 - 20mg of antibody, includes:
Dialysis, digestion, concentration, and purification of the fragments.
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| Available Services |
Typical Yield |
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Cat. No. |
| Fc Fragmentation |
15 - 25% |
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SBCS-71 |
| Fab Fragmentation |
30 - 40% |
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SBCS-72 |
| F(ab')2 Fragmentation |
30 - 40% |
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SBCS-73 |
TESTING SERVICES
SouthernBiotech employs several techniques to evaluate the quantity, quality, and function of purified antibodies,
including: enzyme linked immunosorbent assay (ELISA), Flow Cytometry, Western blotting, SDS-polyacrylamide gel
electrophoresis (SDS-PAGE) and Limulus Amoebocyte Lysate (LAL) assay.
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| Available Services |
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Cat. No. |
| SDS-PAGE Analysis |
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SBCS-62 |
| Isoelectric Focusing (only available in combination w/ SDS-PAGE) 2 Gels |
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SBCS-62I |
| ELISA testing of antigen-specific activity |
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SBCS-60 |
| Western Blotting |
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SBCS-63 |
| Isotype determination by ELISA |
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SBCS-61 |
| Endotoxin testing by LAL Assay |
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SBCS-64 |
| Flow Cytometry Analysis |
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SBCS-65 |
ANTIBODY PURIFICATION AND CHARACTERIZATION
SouthernBiotech uses a variety of methods for purification of antibody from serum, ascites, and culture supernatants including
(NH4)2SO4 salt precipitation, ion exchange and size exclusion chromatography, Protein A and Protein G affinity chromatography,
and custom affinity chromatography. The method employed is dictated by the species, the source material, and
the level of immunoglobulin purity required. The amount of supernatant or serum required per column depends on the concentration
and total milligrams of the solution. Prices are based on the amounts of solution provided:
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Supernatant |
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50mL - 1L |
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Serum |
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50mL - 500mL |
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Ascites |
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5mL- 50mL |
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| Available Services |
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Cat. No. |
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| Ammonium Sulfate Precipitation: |
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SBCS-40 |
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Ammonium sulfate is widely used in isolating both IgM and IgG from a variety
of species. A gel filtration and/or an ion exchange step can be employed to
further purify the immunoglobulin of interest.
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Protein A Purification:
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SBCS-43 |
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High yields of pure IgG can be obtained in a single-step purification using
Protein A or Protein G affinity chromatography, which differ in their ability to
bind antibodies of different origins and IgG subclasses.
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Protein G Purification:
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SBCS-44 |
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High yields of pure IgG can be obtained in a single-step purification using
Protein A or Protein G affinity chromatography, which differ in their ability to
bind antibodies of different origins and IgG subclasses.
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Affinity Purification:
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SBCS-45 |
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Antibodies of extremely high purity and specificity can be obtained using
custom affinity chromatography. A minimum of 10 mg of pure antigen (or
purified peptide) is needed to perform this procedure.
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Size Exclusion/Gel Filtration Chromatography:
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SBCS-42 |
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A single gel matrix or a sequence of size exclusion columns can be used
for purification of IgM and IgG antibodies. The purification strategy employed is
dependent on the immunoglobulin class and the source of the material.
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Ion Exchange Chromatography:
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SBCS-41 |
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This method is useful as an adjunct to ammonium sulfate precipitation for
purifying antibodies from ascites fluid or where protein A and protein G elution
conditions may damage the antibody. It can also be used for removing trace
quantities of DNA contaminants.
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Purification of Antibody from Bioreactor Supernatants:
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SBCS-46 |
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SouthernBiotech has developed a proprietary chromatographic method for
purification of antibodies from cell culture supernatants.
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Aseptic/Low Endotoxin Purification:
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SBCS-48 |
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Purification under aseptic conditions in a dedicated, limited access facility to
generate clean, low-endotoxin antibody preparations.
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Purification of IgY from Chicken Egg Yolks:
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SBCS-47 |
All custom services are accepted and undertaken on a "best efforts" basis only. SouthernBiotech cannot and does
not make any representations or warranties, expressed or implied, including any warranty of merchantability or
fitness for any particular purpose for any material produced or service provided; and, all charges are due and
payable as billed regardless of the outcome of our work. SouthernBiotech shall not in any case be liable for
special, incidental or consequential damages of any kind.
A packaging and shipping charge will be prepaid and added to your invoice. Shipments are normally via Federal
Express or similar carrier unless otherwise mutually agreed to in writing prior to shipment.
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