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CUSTOM SERVICES

IMMUNOCHEMISTRY SERVICES
FRAGMENTATION SERVICES
Antibody fragmentation services for 5 - 20mg of antibody, includes:
Dialysis, digestion, concentration, and purification of the fragments.
Available Services Typical Yield Cat. No.
Fc Fragmentation 15 - 25% SBCS-71
Fab Fragmentation 30 - 40% SBCS-72
F(ab')2 Fragmentation 30 - 40% SBCS-73

TESTING SERVICES
SouthernBiotech employs several techniques to evaluate the quantity, quality, and function of purified antibodies, including: enzyme linked immunosorbent assay (ELISA), Flow Cytometry, Western blotting, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Limulus Amoebocyte Lysate (LAL) assay.

Available Services Cat. No.
SDS-PAGE Analysis SBCS-62
Isoelectric Focusing (only available in combination w/ SDS-PAGE) 2 Gels SBCS-62I
ELISA testing of antigen-specific activity SBCS-60
Western Blotting SBCS-63
Isotype determination by ELISA SBCS-61
Endotoxin testing by LAL Assay SBCS-64
Flow Cytometry Analysis SBCS-65

ANTIBODY PURIFICATION AND CHARACTERIZATION
SouthernBiotech uses a variety of methods for purification of antibody from serum, ascites, and culture supernatants including (NH4)2SO4 salt precipitation, ion exchange and size exclusion chromatography, Protein A and Protein G affinity chromatography, and custom affinity chromatography. The method employed is dictated by the species, the source material, and the level of immunoglobulin purity required. The amount of supernatant or serum required per column depends on the concentration and total milligrams of the solution. Prices are based on the amounts of solution provided:

Supernatant 50mL - 1L
Serum 50mL - 500mL
Ascites 5mL- 50mL
Available Services Cat. No.
Ammonium Sulfate Precipitation: SBCS-40
Ammonium sulfate is widely used in isolating both IgM and IgG from a variety of species. A gel filtration and/or an ion exchange step can be employed to further purify the immunoglobulin of interest.
Protein A Purification: SBCS-43
High yields of pure IgG can be obtained in a single-step purification using Protein A or Protein G affinity chromatography, which differ in their ability to bind antibodies of different origins and IgG subclasses.
Protein G Purification: SBCS-44
High yields of pure IgG can be obtained in a single-step purification using Protein A or Protein G affinity chromatography, which differ in their ability to bind antibodies of different origins and IgG subclasses.
Affinity Purification: SBCS-45
Antibodies of extremely high purity and specificity can be obtained using custom affinity chromatography. A minimum of 10 mg of pure antigen (or purified peptide) is needed to perform this procedure.
Size Exclusion/Gel Filtration Chromatography: SBCS-42
A single gel matrix or a sequence of size exclusion columns can be used for purification of IgM and IgG antibodies. The purification strategy employed is dependent on the immunoglobulin class and the source of the material.
Ion Exchange Chromatography: SBCS-41
This method is useful as an adjunct to ammonium sulfate precipitation for purifying antibodies from ascites fluid or where protein A and protein G elution conditions may damage the antibody. It can also be used for removing trace quantities of DNA contaminants.
Purification of Antibody from Bioreactor Supernatants: SBCS-46
SouthernBiotech has developed a proprietary chromatographic method for purification of antibodies from cell culture supernatants.
Aseptic/Low Endotoxin Purification: SBCS-48
Purification under aseptic conditions in a dedicated, limited access facility to generate clean, low-endotoxin antibody preparations.
Purification of IgY from Chicken Egg Yolks: SBCS-47

TERMS AND CONDITIONS   

All custom services are accepted and undertaken on a "best efforts" basis only. SouthernBiotech cannot and does not make any representations or warranties, expressed or implied, including any warranty of merchantability or fitness for any particular purpose for any material produced or service provided; and, all charges are due and payable as billed regardless of the outcome of our work. SouthernBiotech shall not in any case be liable for special, incidental or consequential damages of any kind.
 
A packaging and shipping charge will be prepaid and added to your invoice. Shipments are normally via Federal Express or similar carrier unless otherwise mutually agreed to in writing prior to shipment.