CUSTOM SERVICES

SouthernBiotech offers a broad range of services for development and production of monoclonal antibodies. Our Hybridoma Development and Monoclonal Antibody Production Services include: immunization, cell fusion, cloning, characterization and production of your monoclonal antibody in vivo in BALB/c, immunocompromized (SCID, Nu/Nu, RAG-/-) mice, or in vitro in flasks or bioreactors. Additional services include antibody purification and conjugation to fluorophores, enzymes and D-biotin. Before initiating an antibody development project, the customer must provide SouthernBiotech with a signed assurance that any customer-supplied material/antigen is mycoplasma-free, non-infectious, non-carcinogenic, and has no known safety concerns to animals or humans.

HYBRIDOMA/MONOCLONAL ANTIBODY DEVELOPMENT SERVICES

Available Services					Cat. No.
Preimmunization Antigen Processing and Modification
			Homogenization of Antigen in Gels or Nitrocellulose Slices		SBCS-19
			Conjugation of antigen for immunization (KLH or other carrier)		SBCS-22K
			Conjugation of antigen to BSA for screening hybridomas		SBCS-22B
			Dialysis of antigen into physiological buffer suitable for injection		SBCS-22D

Phase I:	Immunization and Evaluation of Antibody Titer		SBCS-30 I
	Immunization of 3 BALB/c mice with your supplied and purified antigen. Includes Four (4) immunizations per our standard protocol and daily care. Test bleeds and ELISA data will be provided for your evaluation and decision to continue development.
Phase II:	Fusion, Plating and Screening		SBCS-30 II
	Splenocytes of one of the mice are fused with a selected myeloma cell line. All specific antibody producing colonies are selected by ELISA for expansion. Supernatant from 5 of the best hybridomacontaing wells will be shipped to you for screening. Cryopreservation of up to Five (5) positive clones.
Phase III:	Cloning, Expansion and Cryopreservation		SBCS-30 III
	Subcloning of one positive clone to monoclonality by two rounds of limiting dilution. ELISA screening to detect positive antibody-secreting subclones. Cryopreservation of Ten (10) vials of the hybridoma cell line and isotyping of one monoclonal antibody.
Phase IV:	Amplification		SBCS-30 IV
	Production and shipment of 50mL of monoclonal antibody supernatant from SBCS-30 IV $ 1000 one selected clone.

Additional Hybridoma-Related Services:					Cat. No.
			Subcloning, Expansion and Screening of Additional Clones		SBCS-30S
			Cryopreservation of up to 20 clones per month		SBCS-30S
			Mycoplasma Testing		SBCS-30S

Ascites Production
Ascites Production in Ten (10) BALB/c Mice		SBCS-31
Ascites Production in Twenty (20) BALB/c Mice		SBCS-32
Ascites Production in Fifty (50) BALB/c Mice		SBCS-33
Ascites Production in One Hundred (100) BALB/c Mice		SBCS-34
Ascites Production in Ten (10) Immunocompromised Mice		SBCS-35
Ascites Production in Twenty (20) Immunocompromised Mice		SBCS-36
Ascites Production in Fifty (50) Immunocompromised Mice		SBCS-37
Ascites Production in One Hundred (100) Immunocompromised Mice		SBCS-38

in vitro Production:
Production in 1 Cell Culture Flask
			50 - 100 mL		SBCS-91
Production in 1 Membrane Flask
			30 mL (Concentrated)		SBCS-92
Production in Bioreactor					SBCS-90

To initiate a Custom Contract Service Project:
Contact our Technical Services Department at: (205) 945-1774 or techserv@southernbiotech.com
Toll-free within the US and Canada 1-800-722-2255
A project manager will be assigned and report to you regularly on an agreed schedule.
Discounts and third party arrangements are not accepted in conjunction with SouthernBiotech Custom Services.

TERMS AND CONDITIONS
All custom services are accepted and undertaken on a "best efforts" basis only. SouthernBiotech cannot and does not make any representations or warranties, expressed or implied, including any warranty of merchantability or fitness for any particular purpose for any material produced or service provided; and, all charges are due and payable as billed regardless of the outcome of our work. SouthernBiotech shall not in any case be liable for special, incidental or consequential damages of any kind.
 
A packaging and shipping charge will be prepaid and added to your invoice. Shipments are normally via Federal Express or similar carrier unless otherwise mutually agreed to in writing prior to shipment.