The Antibody Company - SouthernBiotech
 
   

Goat Anti-Mouse Ig, Human ads-HRP

Cat. No.
1010-05  
Size
1.0 mL  
Price (USD)
$115.00 
Qty

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Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, and IgA
Source Pooled antisera from goats hyperimmunized with mouse IgG, IgM, and IgA
Cross Adsorption Human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG, IgM, and IgA covalently linked to agarose
Isotype Goat IgG
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-10
FLISA
Flow Cytometry 15-18
Other referenced applications for relevant formats include -
ELISpot 4,11-14
Immunohistochemistry-Frozen Sections 19
Immunohistochemistry-Paraffin Sections 29
Immunocytochemistry 11,20-23
Immunoblotting 24-28
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Supernatant fractions from Bax-/- mouse embryonic fibroblasts were incubated with caspase-8-cleaved human Bid (tBid) and anti-Bak (Clone 7D10 and Clone 8F8), resolved by electrophoresis, transferred to membrane, and probed with anti-cytochrome c followed by Goat Anti-Mouse Ig, Human ads-HRP (SB Cat. No. 1010-05) and chemiluminescent detection.

    Image from Iyer S, Anwari K, Alsop AE, Yuen WS, Huang DC, Carroll J, et al. Identification of an activation site in Bak and mitochondrial Bax triggered by antibodies. Nat Commun. 2016;7:11734. Figure 1(a)

Supernatant fractions from Bax-/- mouse embryonic fibroblasts were incubated with caspase-8-cleaved human Bid (tBid) and anti-Bak (Clone 7D10 and Clone 8F8), resolved by electrophoresis, transferred to membrane, and probed with anti-cytochrome c followed by Goat Anti-Mouse Ig, Human ads-HRP (SB Cat. No. 1010-05) and chemiluminescent detection.

Image from Iyer S, Anwari K, Alsop AE, Yuen WS, Huang DC, Carroll J, et al. Identification of an activation site in Bak and mitochondrial Bax triggered by antibodies. Nat Commun. 2016;7:11734. Figure 1(a)
 
References
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2. Hochepied T, Wullaert A, Berger FG, Baumann H, Brouckaert P, Steidler L, et al. Overexpression of α1-acid glycoprotein in transgenic mice leads to sensitisation to acute colitis. Gut. 2002;51:398-404. (ELISA)
3. Erlandsson L, Licence S, Gaspal F, Lane P, Corcoran AE, Mårtensson I. Both the pre-BCR and the IL-7Rα are essential for expansion at the pre-BII cell stage in vivo. Eur J Immunol. 2005;35:1969-76. (ELISA)
4. Genestier L, Taillardet M, Mondiere P, Gheit H, Bella C, Defrance T. TLR agonists selectively promote terminal plasma cell differentiation of B cell subsets specialized in thymus-independent responses. J Immunol. 2007;178:7779-86. (ELISA, ELISPOT)
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6. Allan RS, Zueva E, Cammas F, Schreiber HA, Masson V, Belz GT, et al. An epigenetic silencing pathway controlling T helper 2 cell lineage commitment. Nature. 2012;487:249-53. (ELISA)
7. Sage PT, Francisco LM, Carman CV, Sharpe AH. The receptor PD-1 controls follicular regulatory T cells in the lymph nodes and blood. Nat Immunol. 2013;14:152-61. (ELISA)
8. Kruglov AA, Grivennikov SI, Kuprash DV, Winsauer C, Prepens S, Seleznik GM, et al. Nonredundant function of soluble LTα3 produced by innate lymphoid cells in intestinal homeostasis. Science. 2013;342:1243-6. (ELISA)
9. Halemano K, Guo K, Heilman KJ, Barrett BS, Smith DS, Hasenkrug KJ, et al. Immunoglobulin somatic hypermutation by APOBEC3/Rfv3 during retroviral infection. Proc Natl Acad Sci USA. 2014;111:7759-64. (ELISA)
10. Vogelzang A, McGuire HM, Liu SM, Gloss B, Mercado K, Earls P, et al. IL-21 contributes to fatal inflammatory disease in the absence of Foxp3+ T regulatory cells. J Immunol. 2014;192:1404-14. (ELISA)
11. Mandik-Nayak L, Seo S, Sokol C, Potts KM, Bui A, Erikson J. MRL-lpr/lpr mice exhibit a defect in maintaining developmental arrest and follicular exclusion of anti-double-stranded DNA B cells. J Exp Med. 1999;189:1799-814. (ELISPOT, ICC)
12. Rankin AL, Seth N, Keegan S, Andreyeva T, Cook TA, Edmonds J, et al. Selective inhibition of BTK prevents murine lupus and antibody-mediated glomerulonephritis. J Immunol. 2013;191:4540-50. (ELISPOT)
13. Infantino S, Jones SA, Walker JA, Maxwell MJ, Light A, O'Donnell K, et al. The tyrosine kinase Lyn limits the cytokine responsiveness of plasma cells to restrict their accumulation in mice. Sci Signal. 2014;7:ra77. (ELISPOT)
14. Shen P, Roch T, Lampropoulou V, O'Connor RA, Stervbo U, Hilgenberg E, et al. IL-35-producing B cells are critical regulators of immunity during autoimmune and infectious diseases. Nature. 2014;507:366-70. (ELISPOT)
15. Tanaka M, Nagai T, Tsuneyoshi Y, Sunahara N, Matsuda T, Nakamura T, et al. Expansion of a unique macrophage subset in rheumatoid arthritis synovial lining layer. Clin Exp Immunol. 2008;154:38-47. (FC)
16. Hardie DL, Baldwin MJ, Naylor A, Haworth OJ, Hou TZ, Lax S, et al. The stromal cell antigen CD248 (endosialin) is expressed on naive CD8+ human T cells and regulates proliferation. Immunology. 2011;133:288-95. (FC)
17. Zang Y, Martinez L, Fernandez I, Pignac-Kobinger J, Greidinger EL. Conservation of pathogenic TCR homology across class II restrictions in anti-ribonucleoprotein autoimmunity- extended efficacy of T cell vaccine therapy. J Immunol. 2014;192:4093-102. (FC)
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19. Hussell T, Isaacson PG, Crabtree JE, Dogan A, Spencer J. Immunoglobulin specificity of low grade B cell gastrointestinal lymphoma of mucosa-associated lymphoid tissue (MALT) type. Am J Pathol. 1993;142:285-92. (IHC-FS)
20. Shen Y, Meunier L, Hendershot LM. Identification and characterization of a novel endoplasmic reticulum (ER) DnaJ homologue, which stimulates ATPase activity of BiP in vitro and is induced by ER stress. J Biol Chem. 2002;277:15947-56. (ICC)
21. Aasa-Chapman MM, Holuigue S, Aubin K, Wong M, Jones NA, Cornforth D, et al. Detection of antibody-dependent complement-mediated inactivation of both autologous and heterologous virus in primary human immunodeficiency virus type 1 infection. J Virol. 2005;79:2823-30. (ICC)
22. Yi M, Villanueva RA, Thomas DL, Wakita T, Lemon SM. Production of infectious genotype 1a hepatitis C virus (Hutchinson strain) in cultured human hepatoma cells. Proc Natl Acad Sci USA. 2006;103:2310-5. (ICC)
23. Luijten MN, Basten SG, Claessens T, Vernooij M, Scott CL, Janssen R, et al. Birt-Hogg-Dubé syndrome is a novel ciliopathy. Hum Mol Genet. 2013;22:4383-97. (ICC)
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26. Hester SE, Goodfield LL, Park J, Feaga HA, Ivanov YV, Bendor L, et al. Host specificity of ovine Bordetella parapertussis and the role of complement. PLoS One. 2015;10(7):e0130964. (WB)
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