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Rat Anti-Human IL-7-UNLB

Cat. No.
10122-01  
Size
0.5 mg  
Price (USD)
$250.00 
Qty
Add to Cart
Clone BVD10-40F6
Isotype Rat IgG1κ
Isotype Control Mouse IgG1-UNLB
Immunogen E. coli-expressed human IL-7
Specificity Human IL-7
Alternalte Name(s) Interleukin-7, lymphopoietin-1, LP-1, Lpo-1, pre-B cell growth factor, PBGF, thymocyte growth factor
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Capture – Quality tested 1-3
Immunocytochemistry – Reported in literature 4
Neutralization – Reported in literature 1,5

Note – May be paired with the biotinylated clone BVD10-11C10 (SB Cat. No. 10123-08) in a sandwich ELISA
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Standard curve generated with Rat Anti-Human IL-7-UNLB (SB Cat. No. 10122-01; Clone BVD10-40F6) and Rat Anti-Human IL-7-BIOT (SB Cat. No. 10123-08; Clone BVD10-11C10) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05)

Standard curve generated with Rat Anti-Human IL-7-UNLB (SB Cat. No. 10122-01; Clone BVD10-40F6) and Rat Anti-Human IL-7-BIOT (SB Cat. No. 10123-08; Clone BVD10-11C10) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05)
 
References
1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24.(ELISA-Capture, Neut)
2. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
3. Kelly EA, Koziol-White CJ, Clay KJ, Liu LY, Bates ME, Bertics PJ, et al. Potential contribution of IL-7 to allergen-induced eosinophilic airway inflammation in asthma. J Immunol. 2009;182:1404-10. (ELISA-Capture)
4. Bazdar DA, Kalinowska M, Panigrahi S, Sieg SF. Recycled IL-7 can be delivered to neighboring T cells. J Immunol. 2015;194:4698-704. (ICC)
5. Moreau I, Duvert V, Caux C, Galmiche MC, Charbord P, Banchereau J, et al. Myofibroblastic stromal cells isolated from human bone marrow induce the proliferation of both early myeloid and B-lymphoid cells. Blood. 1993;82:2396-405. (Neut)
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