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Goat Anti-Mouse IgM, Human ads-APC

Only %1 left
Cat. No.:
1020-11L,
1020-11S
Goat Anti-Mouse IgM-APC antibody with minimal reactivity to human proteins for use in flow cytometry assays.
As low as $226.00
Size Price (USD) Quantity
0.5 mg (Cat. No. 1020-11L) $352.00
0.25 mg (Cat. No. 1020-11S) $226.00
More Information
Isotype Goat IgG
Isotype Control Goat IgG-APC
Specificity Reacts with the heavy chain of mouse IgM
Source Pooled antisera from goats hyperimmunized with mouse IgM
Cross Adsorption Mouse IgG and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgM covalently linked to agarose
Conjugate APC (Allophycocyanin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 0.5 mL or 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light; Do not freeze
Applications Quality tested applications for relevant formats include -
ELISA 1-11
FLISA
Flow Cytometry 4,7,10,13-17

Other referenced applications for relevant formats include -
ELISpot 2,3,5,6,8-10,12
Immunohistochemistry-Frozen Sections 1,7,18-22
Immunohistochemistry-Paraffin Sections 28
Immunocytochemistry 1,13,23
Electron Microscopy 19
Western Blot 14,16,24
Immunoprecipitation 24,29,30
Purification 26
Stimulation 14,25-27
RRID Number AB_2794210
Gene ID 16019 (Mouse)
Gene ID Symbol Ighm (Mouse)
Gene ID Aliases AI326478; Igh-6; Igh-M; Igh6; Igm; muH
UniProt ID P01872 (Mouse)
UniProt Name IGHM_MOUSE (Mouse)

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  2. 2. Lagrota-Candido J, Vasconcellos R, Cavalcanti M, Bozza M, Savino W, Quirico-Santos T. Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon-γ production. Int J Exp Path. 2002;83:121-32. (ELISA, ELISPOT)
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  6. 6. Patterson HC, Kraus M, Wang D, Shahsafaei A, Henderson JM, Seagal J, et al. Cytoplasmic Igα serine/threonines fine-tune Ig α tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011;187:2853-8. (ELISA, ELISPOT)
  7. 7. Wang JH, Wu Q, Yang P, Li H, Li J, Mountz JD, et al. Type I IFN-dependent CD86high marginal zone precursor B cells are potent T cell costimulators. Arthritis Rheum. 2011;63:1054-64. (ELISA, FC, IHC-FS)
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  11. 11. Pucella JN, Yen W, Kim MV, van der Veeken J, Socci ND, Naito Y, et al. miR-182 is largely dispensable for adaptive immunity: lack of correlation between expression and function. J Immunol. 2015;194:2635-42. (ELISA)
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  13. 13. Eaton-Bassiri AS, Mandik-Nayak L, Seo S, Madaio MP, Cancro MP, Erikson J. Alterations in splenic architecture and the localization of anti-double-stranded DNA B cells in aged mice. Int Immunol. 2000;12:915-26. (FC, ICC)
  14. 14. Geraldes P, Rebrovich M, Herrmann K, Wong J, Jäck H, Wabl M, et al. Ig heavy chain promotes mature B cell survival in the absence of light chain. J Immunol. 2007;179:1659-68. (FC, WB, Stim)
  15. 15. Zhou X, Xiang Y, Ding X, Garrard WT. A new hypersensitive site, HS10, and the enhancers, E3' and Ed, differentially regulate Igκ gene expression. J Immunol. 2012;188:2722-32. (FC)
  16. 16. Alkhatib A, Werner M, Hug E, Herzog S, Eschbach C, Faraidun H, et al. FoxO1 induces Ikaros splicing to promote immunoglobulin gene recombination. J Exp Med. 2012;209:395-406. (FC, WB)
  17. 17. Bai L, Deng S, Reboulet R, Mathew R, Teyton L, Savage PB, et al. Natural killer T (NKT)-B-cell interactions promote prolonged antibody responses and long-term memory to pneumococcal capsular polysaccharides. Proc Natl Acad Sci USA. 2013;110:16097-102. (FC)
  18. 18. Raats CJ, van den Born J, Bakker MA, Oppers-Walgreen B, Pisa BJ, Dijkman HB, et al. Expression of agrin, dystroglycan, and utrophin in normal renal tissue and in experimental glomerulopathies. Am J Pathol. 2000;156:1749-65. (IHC-FS, EM)
  19. 19. Mattsson N, Duzevik EG, Pelsue SC. Expansion of CD22lo B cells in the spleen of autoimmune-prone flaky skin mice. Cell Immunol. 2005;234:124-32. (IHC-FS)
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  21. 21. Simonetti G, Carette A, Silva K, Wang H, De Silva NS, Heise N, et al. IRF4 controls the positioning of mature B cells in the lymphoid microenvironments by regulating NOTCH2 expression and activity. J Exp Med. 2013;210:2887-902. (IHC-FS)
  22. 22. Ren W, Lagerstedt K, Grimsholm O, Stern A, Sun J, Fang Y, et al. Uncoupling of natural IgE production and CD23 surface expression levels. PLoS One. 2013;8(4):e62851. (IHC-FS)
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  24. 24. Elkabetz Y, Shapira I, Rabinovich E, Bar-Nun S. Distinct steps in dislocation of luminal endoplasmic reticulum-associated degradation substrates: roles of endoplamic reticulum-bound p97/Cdc48p and proteasome. J Biol Chem. 2004;279:3980-89. (WB, IP)
  25. 25. Kato I, Takai T, Kudo A. The pre-B cell receptor signaling for apoptosis is negatively regulated by FcγRIIB. J Immunol. 2002;168:629-34. (Stim)
  26. 26. Su Y, Flemming A, Wossning T, Hobeika E, Reth M, Jumaa H. Identification of a pre-BCR lacking surrogate light chain. J Exp Med. 2003;198:1699-706. (Purification, Stim)
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  28. 28. SouthernBiotech published data (IHC-PS)
  29. 29. Koenig P, Nicholls PK, Schmidt FI, Hagiwara M, Maruyama T, Frydman GH, et al. The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice. J BIol Chem. 2014;289:34490-502. (IP)
  30. 30. Ouchida R, Lu Q, Liu J, Li Y, Chu Y, Tsubata T, et al. FcμR interacts and cooperates with the B cell receptor to promote B cell survival. J Immunol. 2015;194:3096-101. (IP)
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