Rat Anti-Mouse IL-6-UNLB

Cat. No.
10207-01  
Size
0.5 mg  
Price (USD)
$200.00 
Clone MP5-20F3
Isotype Rat IgG1κ
Isotype Control Rat IgG1-UNLB
Immunogen COS-expressed mouse IL-6
Specificity Mouse IL-6
Alternalte Name(s) Interleukin-6, hepatocyte stimulating factor, HSF, hybridoma/plasmacytoma growth factor, HPGFB cell stimulating factor-2, BSF-2, cytotoxic T cell differentiation factor, CDF
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Capture – Quality tested 1-7
ELISpot-Capture – Reported in literature 4,8-10
Flow Cytometry – Reported in literature 11-14
Immunohistochemsitry-Frozen Sections – Reported in literature 15-19
Immunohistochemsitry-Paraffin Sections – Reported in literature 20-22
Immunocytochemistry – Reported in literature 3
Immunoprecipitation – Reported in literature 1
Neutralization – Reported in literature 1-3,23
Multiplex-Capture – Reported in literature 5

Note – May be paired with the biotinylated clone MP5-32C11 (SB Cat. No. 10208-08) in a sandwich ELISA
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Standard curve generated with Rat Anti-Mouse IL-6-UNLB (SB Cat. No. 10207-01; Clone MP5-20F3) and Rat Anti-Mouse IL-6-BIOT (SB Cat. No. 10208-08; Clone MP5-32C11) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05)

Standard curve generated with Rat Anti-Mouse IL-6-UNLB (SB Cat. No. 10207-01; Clone MP5-20F3) and Rat Anti-Mouse IL-6-BIOT (SB Cat. No. 10208-08; Clone MP5-32C11) followed by Mouse Anti-BIOT-HRP (SB Cat. No. 6404-05)
 
References
1. Starnes HF Jr, Pearce MK, Tewari A, Yim JH, Zou J, Abrams JS. Anti-IL-6 monoclonal antibodies protect against lethal Escherichia coli infection and lethal tumor necrosis factor-α challenge in mice. J Immunol. 1990;145:4185-91. (Immunogen, ELISA-Capture, IP, Neut)
2. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, Neut)
3. Sander B, Höidén I, Andersson U, Möller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993;166:201-14. (ELISA-Capture, ICC, Neut)
4. Shirai A, Holmes K, Klinman D. Detection and quantitation of cells secreting IL-6 under physiologic conditions in BALB/c mice. J Immunol. 1993;150:793-9. (ELISA-Capture, ELISPOT-Captu