The Antibody Company - SouthernBiotech
 
   

Goat Anti-Mouse IgG2c, Human ads-HRP

Cat. No.
1079-05  
Size
1.0 mL  
Price (USD)
$115.00 
Qty

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Specificity Reacts with the heavy chain of C57BL/6 mouse IgG2c
Source Pooled antisera from goats hyperimmunized with mouse IgG2c
Cross Adsorption Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG2c covalently linked to agarose
Isotype Goat IgG
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation Supplied in a stock solution in 50% glycerol/50% PBS, pH 7.4; No preservative added
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-20
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 21,22,25
ELISpot 5,23
Immunohistochemistry-Paraffin Sections 24
Immunoblotting 25
Depletion 1,25
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2c, Human ads-HRP (SB Cat. No. 1079-05).

ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2c, Human ads-HRP (SB Cat. No. 1079-05).
 
References
1. Lambert SL, Okada CY, Levy R. TCR vaccines against a murine T cell lymphoma: a primary role for antibodies of the IgG2c class in tumor protection. J Immunol. 2004;172:929-36. (ELISA, Depletion)
2. Zhang H, High KA, Wu Q, Yang P, Schlachterman A, Yu S, et al. Genetic analysis of the antibody response to AAV2 and factor IX. Mol Ther. 2005;11:866-74. (ELISA)
3. Magalhaes JG, Fritz JH, Le Bourhis L, Sellge G, Travassos LH, Selvanantham T, et al. Nod2-dependent Th2 polarization of antigen-specific immunity. J Immunol. 2008;181:7925-35. (ELISA)
4. Burrell BE, Lu G, Li XC, Bishop DK. OX40 costimulation prevents allograft acceptance induced by CD40-CD40L blockade. J Immunol. 2009;182:379-90. (ELISA)
5. Swanson CL, Wilson TJ, Strauch P, Colonna M, Pelanda R, Torres RM. Type I IFN enhances follicular B cell contribution to the T cell-independent antibody response. J Exp Med. 2010;207:1485-1500. (ELISA, ELISPOT)
6. Kasturi SP, Skountzou I, Albrecht RA, Koutsonanos D, Hua T, Nakaya HI, et at. Programming the magnitude and persistence of antibody responses with innate immunity. Nature. 2011;470:543-7. (ELISA)
7. Schmitz JM, Durham CG, Schoeb TR, Soltau TD, Wolf KJ, Tanner SM, et al. Helicobacter felis-associated gastric disease in microbiota-restricted mice. J Histochem Cytochem. 2011;59:826-41. (ELISA)
8. Allan RS, Zueva E, Cammas F, Schreiber HA, Masson V, Belz GT, et al. An epigenetic silencing pathway controlling T helper 2 cell lineage commitment. Nature. 2012;487:249-53. (ELISA)
9. Harada Y, Tanaka S, Motomura Y, Harada Y, Ohno S, Ohno S, et al. The 3' enhancer CNS2 is a critical regulator of interleukin-4-mediated humoral immunity in follicular helper T cells. Immunity. 2012;36:188-200. (ELISA)
10. Vogelzang A, McGuire HM, Liu SM, Gloss B, Mercado K, Earls P, et al. IL-21 contributes to fatal inflammatory disease in the absence of Foxp3+ T regulatory cells. J Immunol. 2014;192:1404-14. (ELISA)
11. Halemano K, Guo K, Heilman KJ, Barrett BS, Smith DS, Hasenkrug KJ, et al. Immunoglobulin somatic hypermutation by APOBEC3/Rfv3 during retroviral infection. Proc Natl Acad Sci USA. 2014;111:7759-64. (ELISA)
12. Lam RS, O'Brien-Simpson NM, Lenzo JC, Holden JA, Brammar GC, Walsh KA, et al. Macrophage depletion abates Porphyromonas gingivalis-induced alveolar bone resorption in mice. J Immunol. 2014;193:2349-62. (ELISA)
13. Shen P, Roch T, Lampropoulou V, O'Connor RA, Stervbo U, Hilgenberg E, et al. IL-35-producing B cells are critical regulators of immunity during autoimmune and infectious diseases. Nature. 2014;507:366-70. (ELISA)
14. Soldemo M, Pedersen GK, Karlsson Hedestam GB. HIV-1 Env-specific memory and germinal center B cells in C57BL/6 mice. Viruses. 2014;6:3400-14. (ELISA)
15. Blaauboer SM, Gabrielle VD, Jin L. MPYS/STING-mediated TNF-α, not type I IFN, is essential for the mucosal adjuvant activity of (3'-5')-cyclic-di-guanosine-monophosphate in vivo. J Immunol. 2014;192:492-502. (ELISA)
16. Rowland SL, Riggs JM, Gilfillan S, Bugatti M, Vermi W, Kolbeck R, et al. Early, transient depletion of plasmacytoid dendritic cells ameliorates autoimmunity in a lupus model. J Exp Med. 2014;211:1977-91. (ELISA)
17. Lopez-Saucedo C, Bernal-Reynaga R, Zayas-Jahuey J, Galindo-Gomez S, Shibayama M, Garcia-Galvez C, et al. CD40 ligand deficient C57BL/6 mouse is a potential surrogate model of human X-linked hyper IgM (X-HIGM) syndrome for characterizing immune responses against pathogens. Biomed Res Int. 2015;2015:679850. (ELISA)
18. Teo T, Her Z, Tan JJ, Lum F, Lee WW, Chan Y, et al. Caribbean and La Réunion chikungunya virus isolates differ in their capacity to induce proinflammatory Th1 and NK cell responses and acute joint pathology. J Virol. 2015;89:7955-69. (ELISA)
19. Devera TS, Prusator DK, Joshi SK, Ballard JD, Lang ML. Immunization of mice with anthrax protective antigen limits cardiotoxicity but not hepatotoxicity following lethal toxin challenge. Toxins. 2015;7:2371-84. (ELISA)
20. Blaauboer SM, Mansouri S, Tucker HR, Wang HL, Gabrielle VD, Jin L. The mucosal adjuvant cyclic di-GMP enhances antigen uptake and selectively activates pinocytosis-efficient cells in vivo. Elife. 2015;4:e06670. (ELISA)
21. Bai L, Deng S, Reboulet R, Mathew R, Teyton L, Savage PB, et al. Natural killer T (NKT)-B-cell interactions promote prolonged antibody responses and long-term memory to pneumococcal capsular polysaccharides. Proc Natl Acad Sci USA. 2013;110:16097-102. (FC)
22. Limon JJ, So L, Jellbauer S, Chiu H, Corado J, Sykes SM, et al. mTOR kinase inhibitors promote antibody class switching via mTORC2 inhibition. Proc Natl Acad Sci USA. 2014;111:E5076-85. (FC)
23. Hsu H, Wu Y, Yang P, Wu Q, Job G, Chen J, et al. Overexpression of activation-induced cytidine deaminase in B cells is associated with production of highly pathogenic autoantibodies. J Immunol. 2007;178:5357-65. (ELISPOT)
24. Betz BC, Jordan-Williams KL, Wang C, Kang SG, Liao J, Logan MR, et al. Batf coordinates multiple aspects of B and T cell function required for normal antibody responses. J Exp Med. 2010;207:933-42. (IHC-PS)
25. Esser-von Bieren J, Volpe B, Kulagin M, Sutherland DB, Guiet R, Seitz A, et al. Antibody-mediated trapping of helminth larvae requires CD11b and Fcγ receptor I. J Immunol. 2015;194:1154-63. (FC, WB, Depletion)
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