Rat Anti-Mouse CD40-SPRD

Cat. No.
1645-13  
Size
0.1 mg  
Price (USD)
$280.00 
Clone 1C10
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-SPRD
Immunogen sCD40
Specificity Mouse CD40
Alternalte Name(s) TNFRSF5, Bp50
Description CD40 is a type I cell surface protein belonging to the tumor necrosis factor superfamily of cell surface receptors. In mice it is expressed on B lineage cells, follicular dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zone of secondary lymphoid organs. CD40 first becomes detectable on a subset of small pre-B II cells in bone marrow with the levels of CD40 expression increasing thereafter during B cell maturation. Immature B cells (IgM+IgDloB220lo) express intermediate levels of CD40, whereas mature B cells (IgM+IgDhiB220hi) express high levels. CD40 has a central role in B cell growth and differentiation, and signaling through CD40 in combination with IL-4 reportedly induces immunoglobulin isotype switching and secretion of IgE. The agonistic 1C10 antibody closely resembles gp39/CD40 ligand in its ability to stimulate proliferation of small, resting B lymphocytes in the absence of other cofactors.
Format/Conjugate SPRD (R-phycoerythrin/Cyanine 5)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,7-12
Immunoprecipitation – Reported in literature 1
Activation – Reported in literature 1-4
Blocking – Reported in literature 5,6
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795089
Gene ID
21939 (Mouse)
Gene Symbol
Cd40 (Mouse)
Gene Aliases IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
Uniprot ID
P27512 (Mouse)
Uniprot Name
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD40-SPRD (SB Cat. No. 1645-13).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD40-SPRD (SB Cat. No. 1645-13).
 
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References
1. Heath AW, Wu WW, Howard MC. Monoclonal antibodies to murine CD40 define two distinct functional epitopes. Eur J Immunol. 1994;24:1828-34. (Immunogen, FC, IP, Activ)
2. Randall TD, Heath AW, Santos-Argumedo L, Howard MC, Weissman IL, Lund FE. Arrest of B lymphocyte terminal differentiation by CD40 signaling: mechanism for lack of antibody-secreting cells in germinal centers. Immunity. 1998;8:733-42. (Activ)
3. Nojima T, Hayashi K, Goitsuka R, Nakayama K, Nakayama K, Kitamura D. Double knockout mice show BASH and PKCδ have different epistatic relationships in B cell maturation and CD40-mediated activation. Immunol Lett. 2006;105:48-54. (Activ)
4. Rolph MS, Kaufmann SH. CD40 signaling converts a minimally immunogenic antigen into a potent vaccine against the intracellular pathogen Listeria monocytogenes. J Immunol. 2001;166:5115-21. (Activ)
5. Kluiver JL, Chen CZ. MicroRNAs regulate B-cell receptor signaling-induced apoptosis. Genes Immun. 2012;13:239-44. (Block)
6. Baker RL, Wagner DH Jr, Haskins K. CD40 on NOD CD4 T cells contributes to their activation and pathogenicity. J Autoimmun. 2008;31:385-92. (Block)
7. Brannan CA, Roberts MR. Resident microglia from adult mice are refractory to nitric oxide-inducing stimuli due to impaired NOS2 gene expression. Glia. 2004;48:120-31. (FC)
8. Zaru R, Mollahan P, Watts C. 3-phosphoinositide-dependent kinase 1 deficiency perturbs toll-like receptor signaling events and actin cytoskeleton dynamics in dendritic cells. J Biol Chem. 2007;283:929-39. (FC)
9. Brix S, Lund P, Kjaer TM, Straarup EM, Hellgren LI, Frøkiær H. CD4+ T-cell activation is differentially modulated by bacteria-primed dendritic cells, but is generally down-regulated by n-3 polyunsaturated fatty acids. Immunology. 2010;129:338-50. (FC)
10. Teixeira L, Botelho AS, Mesquita SD, Correia A, Cerca F, Costa R, et al. Plasmacytoid and conventional dendritic cells are early producers of IL-12 in Neospora caninum-infected mice. Immunol Cell Biol. 2010;88:79-86. (FC)
11. Chen Y, Adams E, Regateiro FS, Vaux DJ, Betz AG, Andersen KG, et al. Activation rather than Foxp3 expression determines that TGF-β-induced regulatory T cells out-compete naïve T cells in dendritic cell clustering. Eur J Immunol. 2012;42:1436-48. (FC)
12. Jacobsen J, Haabeth OW, Tveita AA, Schjetne KW, Munthe LA, Bogen B. Naive idiotope-specific B and T cells collaborate efficiently in the absence of dendritic cells. J Immunol. 2014;192:4174-83. (FC)
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