Rat Anti-Mouse CD80-SPRD

Cat. No.
1730-13  
Size
0.1 mg  
Price (USD)
$235.00 
Clone 1G10
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-SPRD
Immunogen Activated 5C2 cells
Specificity Mouse CD80
Alternalte Name(s) B7-1, Ly-53
Description CD80 (B7-1) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed by dendritic cells, monocytes, and peritoneal macrophages. Expression of CD80 on B cells is upregulated after activation by various means including stimulation by LPS, IL-4, and surface Ig receptor crosslinking with peak expression occurring between 48 and 72 hours post-activation. The interaction of CD80 with its ligands, CD28 and CD152 (CTLA-4), provides a potent costimulatory signal for T cells activated through the CD3/T cell receptor complex. The monoclonal antibody 1G10 blocks binding of CTLA-4 to CD80 but it does not block stimulation of T cells by natural antigen-presenting cells.
Format/Conjugate SPRD (R-phycoerythrin/Cyanine 5)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-12
Immunoprecipitation – Reported in literature 2
Blocking – Reported in literature 1-3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795209
Gene ID
12519 (Mouse)
Gene Symbol
Cd80 (Mouse)
Gene Aliases B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
Uniprot ID
Q00609 (Mouse)
Uniprot Name
  • DBA/2 mouse mastocytoma cell line P815 and mB7.1-Ig transfected P815 cells were stained with Rat Anti-Mouse CD80-SPRD (SB Cat. No. 1730-13).

DBA/2 mouse mastocytoma cell line P815 and mB7.1-Ig transfected P815 cells were stained with Rat Anti-Mouse CD80-SPRD (SB Cat. No. 1730-13).
 
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References
1. Powers GD, Faherty DA, Connaughton SE, Biondi DA, Godfrey DI, Gault A, et al. Expression and functional analysis of murine B7 delineated by a novel monoclonal antibody. Cell Immunol. 1994;153:298-311. (Immunogen, Block, FC)
2. Chen C, Faherty DA, Gault A, Connaughton SE, Powers GD, Godfrey DI, et al. Monoclonal antibody 2D10 recognizes a novel T cell costimulatory molecule on activated murine B lymphocytes. J Immunol. 1994;152:2105-14. (IP, FC, Block)
3. Nabavi N, Freeman GJ, Gault A, Godfrey D, Nadler LM, Glimcher LH. Signalling through the MHC class II cytoplasmic domain is required for antigen presentation and induces B7 expression. Nature. 1992;360:266-8. (FC, Block)
4. Cheng X, Wang C, Qian G, Zhu B. CD80, but not CD86 were up-regulated on the spleen-derived dendritic cells from OVA-sensitized and challenged BALB/c mice. Immunol Lett. 2003;89:31-38. (FC)
5. Lee S, Bar-Haim E, Machlenkin A, Goldberger O, Volovitz I, Vadai E, et al. In vivo rejection of tumor cells dependent on CD8 cells that kill independently of perforin and FasL. Cancer Gene Ther. 2004;11:237-48. (FC)
6. He W, Hu Z, Liu F, Feng X, Zou P. In vitro co-stimulation of anti-tumor activity by soluble B7 molecules. Acta Biochim Pol. 2006;53:807-13. (FC)
7. Molling JW, Moreno M, van der Vliet HJ, von Blomberg BM, van den Eertwegh AJ, Scheper RJ, et al. Generation and sustained expansion of mouse spleen invariant NKT cell lines with preserved cytokine releasing capacity. J Immunol Methods. 2007;322:70-81. (FC)
8. Xu H, Liew LN, Kuo IC, Huang CH, Goh DL, Chua KY. The modulatory effects of lipopolysaccharide-stimulated B cells on differential T-cell polarization. Immunology. 2008;125:218-28. (FC)
9. Brix S, Lund P, Kjaer TM, Straarup EM, Hellgren LI, Frøkiær H. CD4+ T-cell activation is differentially modulated by bacteria-primed dendritic cells, but is generally down-regulated by n-3 polyunsaturated fatty acids. Immunology. 2010;129:338-50. (FC)
10. Jacobsen JT, Lunde E, Sundvold-Gjerstad V, Munthe LA, Bogen B. The cellular mechanism by which complementary Id+ and anti-Id antibodies communicate: T cells integrated into idiotypic regulation. Immunol Cell Biol. 2010;88:515-22. (FC)
11. Grodeland G, Mjaaland S, Roux KH, Fredriksen AB, Bogen B. DNA vaccine that targets hemagglutinin to MHC class II molecules rapidly induces antibody-mediated protection against influenza. J Immunol. 2013;191:3221-31. (FC)
12. Li J, Liu Y, Gao X, Gao X, Cai H. TLR2 and TLR4 signaling pathways are required for recombinant Brucella abortus BCSP31-induced cytokine production, functional upregulation of mouse macrophages, and the Th1 immune response in vivo and in vitro. Cell Mol Immunol. 2014;11:477-94. (FC)
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