Rat Anti-Mouse Ly-6A/E-FITC

Cat. No.
1755-02  
Size
0.5 mg  
Price (USD)
$245.00 
Clone D7
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-FITC
Immunogen IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L
Specificity Mouse Ly-6A/E (Ly-6A.2 and Ly-6E.1)
Alternalte Name(s) Sca-1
Description Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. Studies with the monoclonal antibody D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,3-7
Immunohistochemistry-Frozen Sections – Reported in literature 3
Immunohistochemistry-Paraffin Sections – Reported in literature 2
Immunoprecipitation – Reported in literature 1,3
Western Blot – Reported in literature 1,3
Costimulation – Reported in literature 4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795244
Gene ID
110454 (Mouse)
17069 (Mouse)
Gene Symbol
Ly6a (Mouse)
Ly6e (Mouse)
Gene Aliases TAP; Sca1; Sca-1; Ly-6A.2; Ly-6A/E; Ly-6E.1 Ly67; RIG-E; Sca-2; TSA-1; Tsa1
Uniprot ID
P05533 (Mouse)
Q64253 (Mouse)
Uniprot Name
  • Con-A stimulated C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse Ly-6A/E-FITC (SB Cat. No. 1755-02).

Con-A stimulated C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse Ly-6A/E-FITC (SB Cat. No. 1755-02).
 
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References
1. Ortega G, Korty PE, Shevach EM, Malek TR. Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity. J Immunol. 1986;137:3240-6. (Immunogen, FC, IP, WB)
2. van Bragt MP, Ciliberti N, Stanford WL, de Rooij DG, van Pelt AM. LY6A/E (SCA-1) expression in the mouse testis. Biol Reprod. 2005;73:634-8. (IHC-PS)
3. English A, Kosoy R, Pawlinski R, Bamezai A. A monoclonal antibody against the 66-kDa protein expressed in mouse spleen and thymus inhibits Ly-6A.2-dependent cell-cell adhesion. J Immunol. 2000;165:3763-71. (FC, IP, WB, IHC-FS)
4. Codias EK, Malek TR. Regulation of B lymphocyte responses to IL-4 and IFN-γ by activation through Ly-6A/E molecules. J Immunol. 1990;144:2197-204. (Costim, FC)
5. George J, Afek A, Abashidze A, Shmilovich H, Deutsch V, Kopolovich J, et al. Transfer of endothelial progenitor and bone marrow cells influences atherosclerotic plaque size and composition in apolipoprotein E knockout mice. Arterioscler Thromb Vasc Biol. 2005;25:2636-41. (FC)
6. Pevsner-Fischer M, Morad V, Cohen-Sfady M, Rousso-Noori L, Zanin-Zhorov A, Cohen S, et al. Toll-like receptors and their ligands control mesenchymal stem cell functions. Blood. 2007;109:1422-32. (FC)
7. Filip S, Mokrý J, Vávrová J, Šinkorová Z, Mičuda S, Šponer P, et al. The peripheral chimerism of bone marrow-derived stem cells after transplantation: regeneration of gastrointestinal tissues in lethally irradiated mice. J Cell Mol Med. 2014;18:832-43. (FC)