Rat Anti-Mouse CD122-UNLB
Cat. No.
1905-01
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Size
0.5 mg
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Price (USD)
$192.00
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Clone |
5H4
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Isotype |
Rat (Lewis) IgG2aκ
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Isotype Control |
Rat IgG2a-UNLB
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Immunogen |
Rat myeloma YB2/0 transfected with truncated IL-2Rβ cDNA (YB2/0-mβt-28)
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Specificity |
Mouse CD122
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Alternalte Name(s) |
IL-2Rβ, IL-15 receptor β chain
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Description |
The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the α chain which binds IL-2 with low affinity; (ii) the β chain that binds IL-2 with high affinity; and (iii) the common γ chain (γc) that does not bind IL-2. The high affinity receptor complex is an α/β/γ heterotrimer with a Kd of 1.3 x 10-11 M. In mouse spleen, CD122 is expresed on ~30% of CD8+ cells and all NK cells but <1% of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8- double negative cells. Cytoplasmic regions of the IL-2R β chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes. The monoclonal antibody 5H4 does not block IL-2 binding.
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Format/Conjugate |
UNLB (Unconjugated)
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Buffer Formulation |
Borate buffered saline, pH 8.2
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Concentration |
0.5 mg/mL
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Volume |
1.0 mL
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Storage & Handling |
2-8°C
Please refer to product specific SDS
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Applications |
Applications for relevant formats of this clone include - Flow Cytometry – Quality tested 1,2 Immunoprecipitation – Reported in literature 1,2
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Recommended Dilutions |
Please refer to product specific Technical Bulletin
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RRID |
AB_2795479
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Gene ID |
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Gene Symbol |
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Gene Aliases |
p70; CD122; IL15Rbeta; Il-2Rbeta; IL-15Rbeta; Il-2/15Rbeta
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Uniprot ID |
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Uniprot Name |
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-
BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD112-FITC (SB Cat. No. 1905-02) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).
BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD112-FITC (SB Cat. No. 1905-02) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).
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References
1. | Malek TR, Furse RK, Fleming ML, Fadell AJ, He Y. Biochemical identity and characterization of the mouse interleukin-2 receptor β and γc subunits. J Interferon Cytokine Res. 1995;15:447-54. (Immunogen, FC, IP) | 2. | Furse RK, Malek TR. Selection of internalization-deficient cells by interleukin-2-Pseudomonas exotoxin chimeric protein: the cytoplasmic domain of the interleukin-2 receptor β chain does not contribute to internalization of interleukin-2. Eur J Immunol. 1993;23:3181-8. (FC, IP) |
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