Rat Anti-Mouse CD122-UNLB

Cat. No.
1905-01  
Size
0.5 mg   		Price (USD)
$192.00 
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Clone 5H4
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-UNLB
Immunogen Rat myeloma YB2/0 transfected with truncated IL-2Rβ cDNA (YB2/0-mβt-28)
Specificity Mouse CD122
Alternalte Name(s) IL-2Rβ, IL-15 receptor β chain
Description The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the α chain which binds IL-2 with low affinity; (ii) the β chain that binds IL-2 with high affinity; and (iii) the common γ chain (γc) that does not bind IL-2. The high affinity receptor complex is an α/β/γ heterotrimer with a Kd of 1.3 x 10-11 M. In mouse spleen, CD122 is expresed on ~30% of CD8+ cells and all NK cells but <1% of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8- double negative cells. Cytoplasmic regions of the IL-2R β chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes. The monoclonal antibody 5H4 does not block IL-2 binding.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,2
Immunoprecipitation – Reported in literature 1,2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795479
Gene ID
16185 (Mouse)
Gene Symbol
Il2rb (Mouse)
Gene Aliases p70; CD122; IL15Rbeta; Il-2Rbeta; IL-15Rbeta; Il-2/15Rbeta
Uniprot ID
P16297 (Mouse)
Uniprot Name
IL2RB_MOUSE 
  • Rat Anti-Mouse CD122-FITC
    BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD112-FITC (SB Cat. No. 1905-02) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD112-FITC (SB Cat. No. 1905-02) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).
 
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References
1. Malek TR, Furse RK, Fleming ML, Fadell AJ, He Y. Biochemical identity and characterization of the mouse interleukin-2 receptor β and γc subunits. J Interferon Cytokine Res. 1995;15:447-54. (Immunogen, FC, IP)
2. Furse RK, Malek TR. Selection of internalization-deficient cells by interleukin-2-Pseudomonas exotoxin chimeric protein: the cytoplasmic domain of the interleukin-2 receptor β chain does not contribute to internalization of interleukin-2. Eur J Immunol. 1993;23:3181-8. (FC, IP)