Goat F(ab')2 Anti-Human Lambda-PE

Cat. No.
2072-09  
Size
0.25 mg  
Price (USD)
$295.00 
Specificity Reacts with human λ light chains
Source Pepsin digest of Goat Anti-Human Lambda (SB Cat. No. 2070)
Cross Adsorption Human κ light chains; may react with λ light chains from other species
Isotype Goat F(ab')2 IgG
Isotype Control Goat F(ab')2 IgG-PE
Format/Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Concentration 0.25 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Flow Cytometry 2,3
Other referenced applications for relevant formats include -
Western Blot 1
Stimulation 4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795770
  • CD19+ human B-lymphocytes were stained with Goat F(ab’)2 Anti-Human Lambda-PE (SB Cat. 2072-09) and Goat F(ab’)2 Anti-Human Kappa-FITC (SB Cat. No. 2062-02).
  • FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Human Lambda-PE (SB Cat. No. 2072-09).

CD19+ human B-lymphocytes were stained with Goat F(ab’)2 Anti-Human Lambda-PE (SB Cat. 2072-09) and Goat F(ab’)2 Anti-Human Kappa-FITC (SB Cat. No. 2062-02).
 

FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Human Lambda-PE (SB Cat. No. 2072-09).
 
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References
1. Macmillan H, Strohman MJ, Ayyangar S, Jiang W, Rajasekaran N, Spura A, et al. The MHC class II cofactor HLA-DM interacts with Ig in B cells. J Immunol. 2014;193:2641-50. (WB)
2. Labrijn AF, Buijsse AO, van den Bremer ET, Verwilligen AY, Bleeker WK, Thorpe SJ, et al. Therapeutic IgG4 antibodies engage in Fab-arm exchange with endogenous human IgG4 in vivo. Nat Biotechnol. 2009;27:767-71. (FC)
3. de Jongste AH, Kraan J, van den Broek PD, Brooimans RA, Bromberg JE, van Montfort KA. Use of TransFix™ cerebrospinal fluid storage tubes prevents cellular loss and enhances flow cytometric detection of malignant hematological cells after 18 hours of storage. Cytometry B Clin Cytom. 2014;86:272-9. (FC)
4. Habib T, Funk A, Rieck M, Brahmandam A, Dai X, Panigrahi AK, et al. Altered B cell homeostasis is associated with type I diabetes and carriers of the PTPN22 allelic variant. J Immunol. 2012;188:487-96. (Stim)