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  • Goat Anti-Rabbit IgG Fab, Mouse/Human SP ads-AP

Goat Anti-Rabbit IgG Fab, Mouse/Human SP ads-AP

Cat. No.:
4058-04
Goat Anti-Rabbit IgG Fab-AP antibody with minimal reactivity to mouse and human serum proteins for use in ELISA assays.
$154.00
Size Price (USD) Quantity
1.0 mL $154.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control 0109-04
Specificity Reacts with the Fab region of rabbit IgG
Source Pooled antisera from goats hyperimmunized with rabbit IgG
Cross Adsorption Rabbit IgG Fc; mouse and human serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rabbit IgG Fab covalently linked to agarose
Conjugate AP (Alkaline Phosphatase)
Buffer Formulation 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0
Clonality Polyclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2895789

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  2. ELISA plate was coated with purified rabbit IgG Fab and IgG Fc. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit Light Chain-AP (SB Cat. No. 4060-04).
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  3. Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.
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  5. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-AP (SB Cat. No. 4050-04).
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  6. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  7. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG-AP (SB Cat. No. 4030-04).
    Goat Anti-Rabbit IgG-AP
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  8. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit Ig, Human ads-AP (SB Cat. No. 4010-04).
    Goat Anti-Rabbit Ig, Human ads-AP
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  9. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG(H+L), Human ads-AP (SB Cat. No. 4051-04).
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  10. ELISA plate was coated with serially diluted Rabbit IgM-UNLB (SB Cat. No. 0113-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) and Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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