Rabbit Anti-Mouse IgG(H+L), Human ads-Alexa Fluor® 647

Cat. No.
6175-31  
Size
1.0 mg  
Price (USD)
$140.00 
Specificity Reacts with the heavy and light chains of mouse IgG and the light chains of mouse IgM and IgA
Source Pooled antisera from rabbits hyperimmunized with mouse IgG
Cross Adsorption Human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG covalently linked to agarose
Isotype Rabbit IgG
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1
FLISA
Flow Cytometry 2
Other referenced applications for relevant formats include -
Western Blot 3-5
Immunocytochemistry 6
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796253
  • FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Mouse IgG(H+L), Human ads-AF647 (SB Cat. No. 6175-31).

FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Mouse IgG(H+L), Human ads-AF647 (SB Cat. No. 6175-31).
 
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References
1. Stone CB. Structural and biochemical characterization of the chlamydia pneumoniae type III secretion system [thesis]. Hamilton (Canada): McMaster University; 2012. (ELISA)
2. Melcher M, Muhle RA, Henrich PP, Kraemer SM, Avril M, Vigan-Womas I, et al. Identification of a role for the PfEMP1 semi-conserved head structure in protein trafficking to the surface of Plasmodium falciparum infected red blood cells. Cell Microbiol. 2010;12:1446-62. (FC)
3. Kaneko K, Miyabe Y, Takayasu A, Fukuda S, Miyabe C, Ebisawa M, et al. Chemerin activates fibroblast-like synoviocytes in patients with rheumatoid arthritis. Arthritis Res Ther. 2011;13:R158. (WB)
4. Gao W, Zhang C, Feng Y, Chen G, Wen S, Huangfu H, et al. Fascin-1, ezrin and paxillin contribute to the malignant progression and are predictors of clinical prognosis in laryngeal squamous cell carcinoma. PLoS One. 2012;7(11):e50710. (WB)
5. Zhao L, Chen J, Cheng L, Wang X, Du J, Wang F, et al. Effects of Enterococcus faecalis lipoteichoic acid on receptor activator of nuclear factor-κB ligand and osteoprotegerin expression in periodontal ligament fibroblasts. Int Endod J. 2014;47:163-72. (WB)
6. SouthernBiotech published data (ICC)
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