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  • Goat Anti-Rabbit IgM, Multi-Species SP ads-HRP

Goat Anti-Rabbit IgM, Multi-Species SP ads-HRP

Cat. No.:
4021-05
Goat Anti-Rabbit IgM-HRP antibody with minimal reactivity to human, mouse, rat, bovine, hamster, goat, sheep, chicken, and guinea pig serum proteins for use in ELISA assays.
$176.00
Size Price (USD) Quantity
1.0 mL $176.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control 0109-05
Specificity Reacts with the heavy chain of rabbit IgM
Source Pooled antisera from goats hyperimmunized with rabbit IgM
Cross Adsorption Rabbit IgG; human, mouse, rat, bovine, hamster, goat, sheep, chicken, and guinea pig serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rabbit IgM covalently linked to agarose
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality Polyclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA

RRID Number AB_2895774

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  2. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05).
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  3. Reduced (Lane 1) and non-reduced rabbit IgG (Lane 2) was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit Light Chain-HRP (SB Cat. No. 4060-05). Light chains were visualized using chemiluminescent detection.
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  4. ELISA plate was coated with serially diluted Rabbit IgM-UNLB (SB Cat. No. 0113-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) and Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  6. Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01). Proteins were visualized using Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 4050-05) secondary antibody and chemiluminescent detection.
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