His-tags are a series of consecutive histidine residues, commonly six, which are typically fused to the N- or C-terminal end of
recombinant proteins. Its small size minimizes interference with the structure and folding of the protein. His-tag residues bind
to several transition metal ions such as nickel, cobalt, and copper which, when immobilized on beads or a resin, allows for the
simple purification of His-tagged proteins.
SouthernBiotech’s His-tag antibody (Clone SB194b) offers a reliable method to monitor purification as well as perform purification
of His-tagged proteins without the use of a protein-specific antibody. Additionally, Clone SB194b is useful for the detection
of N- and C-terminal as well as exposed internal His-tag residues of 6 or more in western blotting, immunoprecipitation, ELISA / FLISA, flow cytometry, and
immunocytochemistry applications. Directly conjugated options allow for detection without the use of a secondary antibody.