Rat Anti-Mouse IgG2a-Alexa Fluor® 488

Cat. No.
1155-30  
Size
0.1 mg  
Price (USD)
$190.00 
Clone SB84a
Isotype Rat (Sprague Dawley) IgG1κ
Isotype Control Rat IgG1-Alexa Fluor® 488
Immunogen Mouse IgG2a hybridoma
Specificity Mouse IgG2a
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,2
ELISA – Quality tested 3-9
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 10
Immunocytochemistry 11
Western Blot 11
Multiplex – Reported in literature 12
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794652
  • FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgG2a-AF488 (SB Cat. No. 1155-30).

FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgG2a-AF488 (SB Cat. No. 1155-30).
 
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References
1. Mandt T, Song Y, Scharenberg AM, Sahni J. SLC41A1 Mg2+ transport is regulated via Mg2+-dependent endosomal recycling through its N-terminal cytoplasmic domain. Biochem J. 2011;439:129-39. (FC)
2. Peters A, Pitcher LA, Sullivan JM, Mitsdoerffer M, Acton SE, Franz B, et al. Th17 cells induce ectopic lymphoid follicles in central nervous system tissue inflammation. Immunity. 2011;35:986-96. (FC)
3. Su B, Wang J, Zhao G, Wang XJ, Li J, Wang B. Sequential administration of cytokine genes to enhance cellular immune responses and CD4+ T memory cells during DNA vaccination. Hum Vaccin Immunother. 2012;8:1659-67. (ELISA)
4. Pichler U, Hauser M, Hofer H, Himly M, Hoflehner E, Steiner M, et al. Allergen hybrids - next generation vaccines for Fagales pollen immunotherapy. Clin Exp Allergy. 2014;44:438-49. (ELISA)
5. Sousa LM, Carneiro MB, Resende ME, Martins LS, Dos Santos LM, Vaz LG, et al. Neutrophils have a protective role during early stages of Leishmania amazonensis infection in BALB/c mice. Parasite Immunol. 2014;36:13-31. (ELISA)
6. Chen M, Hong MJ, Sun H, Wang L, Shi X, Gilbert BE, et al. Essential role for autophagy in the maintenance of immunological memory against influenza infection. Nat Med. 2014;20:503-10. (ELISA)
7. Joshi VB, Adamcakova-Dodd A, Jing X, Wongrakpanich A, Gibson-Corley KN, Thorne PS, et al. Development of a poly (lactic-co-glycolic acid) particle vaccine to protect against house dust mite induced allergy. AAPS J. 2014;16:975-85. (ELISA)
8. Patel JM, Vartabedian VF, Kim M, He S, Kang S, Selvaraj P. Influenza virus-like particles engineered by protein transfer with tumor-associated antigens induces protective antitumor immunity. Biotechnol Bioeng. 2015;112:1102-10. (ELISA)
9. Zuest R, Valdes I, Skibinski D, Lin Y, Toh YX, Chan K, et al. Tetravalent dengue DIIIC protein together with alum and ODN elicits a Th1 response and neutralizing antibodies in mice. Vaccine. 2015;33:1474-82. (ELISA)
10. Liu Z. Immunofluorescent staining of frozen murine kidney sections. Bio-protocol. 2011;1:e65. (IHC-FS)
11. SouthernBiotech unpublished data (ICC, WB)
12. Brummelman J, Helm K, Hamstra H, van der Ley P, Boog CJ, Han WG, et al. Modulation of the CD4+ T cell response after acellular pertussis vaccination in the presence of TLR4 ligation. Vaccine. 2015;33:1483-91. (Multiplex)
Alexa Fluor® is a registered trademark of Life Technologies Corporation