Rat Anti-Mouse CD25-SPRD

Cat. No.
1600-13  
Size
0.1 mg  
Price (USD)
$225.00 
Clone 3C7
Isotype Rat (Lewis) IgG2bκ
Isotype Control Rat IgG2b-SPRD
Immunogen IL-2 dependent BALB/c helper T cell clone HT2
Specificity Mouse CD25
Alternalte Name(s) IL-2Rα, p55, IL-2 receptor α chain, Ly-43, Tac, IL-2-RA
Description The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity α chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells, and monocytes of all mouse strains tested. Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated. CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD. It is expressed at a higher level on CD4+CD8+ thymocytes. It is also expressed on cultured epidermal Langerhans cells. The biochemical features of murine CD25 have been characterized in detail. The 3C7 monoclonal antibody reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4. 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone.
Format/Conjugate SPRD (R-phycoerythrin/Cyanine 5)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,4
Immunohistochemistry-Frozen Sections – Reported in literature 5,6
Immunoprecipitation – Reported in literature 1
Blocking – Reported in literature 1,3
Neutralization – Reported in literature 2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795017
  • C57BL/6 mouse cytotoxic T lymphocyte cell line CTLL-2 was stained with Rat Anti-Mouse CD25-SPRD (SB Cat. No. 1600-13).

C57BL/6 mouse cytotoxic T lymphocyte cell line CTLL-2 was stained with Rat Anti-Mouse CD25-SPRD (SB Cat. No. 1600-13).
 
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References
1. Ortega G, Robb RJ, Shevach EM, Malek TR. The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells. J Immunol. 1984;133:1970-5. (Immunogen, Block, IP, FC)
2. de Goër de Herve MG, Jaafoura S, Vallée M, Taoufik Y. FoxP3+ regulatory CD4 T cells control the generation of functional CD8 memory. Nat Commun. 2012;3:986. (Neut)
3. Malek TR, Ortega G, Jakway JP, Chan C, Shevach EM. The murine IL 2 receptor. II. Monoclonal anti-IL 2 receptor antibodies as specific inhibitors of T cell function in vitro. J immunol. 1984;133:1976-82. (Block)
4. Li H, Matte-Martone C, Tan HS, Venkatesan S, McNiff J, Demetris AJ, et al. Graft-versus-host disease is independent of innate signaling pathways triggered by pathogens in host hematopoietic cells. J Immunol. 2011;186:230-41. (FC)
5. Klug DB, Carter C, Crouch E, Roop D, Conti CJ, Richie ER. Interdependence of cortical thymic epithelial cell differentiation and T-lineage commitment. Proc Natl Acad Sci USA. 1998;95:11822-7. (IHC-FS)
6. Comes A, Rosso O, Orengo AM, Di Carlo E, Sorrentino C, Meazza R, et al. CD25+ regulatory T cell depletion augments immunotherapy of micrometastases by an IL-21-secreting cellular vaccine. J Immuno. 2006;176:1750-8. (IHC-FC)
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