Rat Anti-Mouse CD71-BIOT

Cat. No.
1720-08  
Size
0.5 mg  
Price (USD)
$275.00 
Clone RI7217
Isotype Rat (BDIX) IgG2aκ
Isotype Control Rat IgG2a-BIOT
Immunogen DMSO induced Friend erythroleukemia 745.6
Specificity Mouse CD71
Alternalte Name(s) T9, transferrin receptor, TfR
Description CD71, a type II transmembrane glycoprotein expressed on the cell surface as a homodimer, is the transferrin receptor. It is essential for the growth of normal and neoplastic cells. It is expressed at low levels on resting B and T lymphocytes but is upregulated during responses to antigens and mitogens presumably reflecting the iron dependence of proliferation. Its expression declines with maturation and differentiation. CD71 plays a critical role in cell proliferation by controlling the supply of iron which is essential for many metabolic pathways through the binding and endocytosis of transferrin, the major iron-carrying protein. It may also be involved in signal transduction via its association with the T cell receptor ζ chain. The monoclonal antibody RI7217 inhibits cell proliferation in vitro.
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,2,4,5
Immunocytochemistry – Reported in literature 5
Immunoprecipitation – Reported in literature 2
Western Blot – Reported in literature 9
Blocking – Reported in literature 2,3
Separation – Reported in literature 6
Drug Delivery – Reported in literature 7,8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795192
  • Mouse pre-B cell line 18-81 was stained with Rat Anti-Mouse CD71-BIOT (SB Cat. No. 1720-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

Mouse pre-B cell line 18-81 was stained with Rat Anti-Mouse CD71-BIOT (SB Cat. No. 1720-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
 
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References
1. Lesley J, Hyman R, Schulte R, Trotter J. Expression of transferrin receptor on murine hematopoietic progenitors. Cell Immunol. 1984;83:14-25. (Immunogen, FC)
2. Lesley JF, Schulte RJ. Inhibition of cell growth by monoclonal anti-transferrin receptor antibodies. Mol Cell Biol. 1985;5:1814-21. (FC, IP, Block)
3. Kemp JD, Thorson JA, Gomez F, Snith KM, Cowdery JS, Ballas ZK. Inhibition of lymphocyte activation with anti-transferrin receptor Mabs: A comparison of three reagents and further studies of their range of effects and mechanism of action. Cell Immunol. 1989;122:218-30. (Block)
4. van der Weyden L, Arends MJ, Rust AG, Poulogiannis G, McIntyre RE, Adams DJ. Increased tumorigenesis associated with loss of the tumor suppressor gene Cadm1. Mol Cancer. 2012;11:29. (FC)
5. Wang J, Wagner-Britz L, Bogdanova A, Ruppenthal S, Wiesen K, Kaiser E, et al. Morphologically homogeneous red blood cells present a heterogeneous response to hormonal stimulation. PloS One. 2013;8(6):e67697. (ICC, FC)
6. Romero JR, Suzuka SM, Nagel RL, Fabry ME. Expression of HbC and HbS, but not HbA, results in activation of K-Cl cotransport activity in transgenic mouse red cells. Blood. 2004;103:2384-90. (Sep)
7. Inoue S, Patil R, Portilla-Arias J, Ding H, Konda B, Espinoza A, et al. Nanobiopolymer for direct targeting and inhibition of EGFR expression in triple negative breast cancer. PLoS One. 2012;7(2):e31070. (Drug Delivery)
8. Inoue S, Ding H, Portilla-Arias J, Hu J, Konda B, Fujita M, et al. Polymalic acid-based nanobiopolymer provides efficient systemic breast cancer treatment by inhibiting both HER2/neu receptor synthesis and activity. Cancer Res. 2011;71:1454-64. (Drug Delivery)
9. Fiani ML, Beitz J, Turvy D, Blum JS, Stahl PD. Regulation of mannose receptor synthesis and turnover in mouse J774 macrophages. J Leukoc Biol. 1998;64:85-91. (WB)