Hamster Anti-Mouse TCRγδ-Alexa Fluor® 488

Cat. No.
1780-30  
Size
0.1 mg  
Price (USD)
$190.00 
Clone UC7-13D5
Isotype Hamster (Armenian) IgG3
Isotype Control Hamster IgG-Alexa Fluor® 488
Immunogen Mouse T cell clone G8
Specificity Mouse TCRγδ
Description The TCRγδ heterodimer of the CD3/T cell receptor complex is clonotypic and consists of Ig-like variable and constant domains. It is expressed during thymopoiesis and on the small subpopulation of γδ TCR-expressing T lymphocytes in the periphery. The majority of T cells present in some epithelial tissues are γδ+ and have limited receptor diversity. The TCRγδ heterodimer recognizes peptide antigen bound to MHC antigens and subsequent signal transduction mediated by the invariant chains leads to T cell activation. The monoclonal antibody UC7-13D5 is specific for the γδ heterodimer and plate-bound UC7-13D5 activates γδ TCR-bearing cells. This antibody does not react with αβ TCR-expressing T cells.
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 6
Immunoprecipitation – Reported in literature 1,2
Costimulation – Reported in literature 1,5
Depletion – Reported in literature 3,4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795277
  • BALB/c mouse thymocytes were stained with Hamster Anti-Mouse TCRγδ-AF488 (SB Cat. No. 1780-30) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).

BALB/c mouse thymocytes were stained with Hamster Anti-Mouse TCRγδ-AF488 (SB Cat. No. 1780-30) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).
 
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References
1. Bluestone JA, Cron RQ, Barrett TA, Houlden B, Sperling AI, Dent A, et al. Repertoire development and ligand specificity of murine TCRγδ cells. Immunol Rev. 1991;120:5-33. (IP, Costim)
2. Belles C, Kuhl AL, Donoghue AJ, Sano Y, O'Brien RL, Born W, et al. Bias in the γδ T cell response to Listeria monocytogenes. Vδ6.3+ cells are a major component of the γδ T cell response to Listeria monocytogenes. J Immunol. 1996;156:4280-9. (IP)
3. Hiromatsu K, Yoshikai Y, Matsuzaki G, Ohga S, Muramori K, Matsumoto K, et al. A protective role of gamma/delta T cells in primary infection with Listeria monocytogenes in mice. J Exp Med. 1992;175:49-56. (Depletion)
4. Shibata K, Yamada H, Hara H, Kishihara K, Yoshokai Y. Resident Vδ1+ γδ T cells control early infiltration of neutrophils after Escherichia coli infection via IL-17 production. J Immunol. 2007;178:4466-72. (Depletion)
5. Sperling AI, Linsley PS, Barrett TA, Bluestone JA. CD28-mediated costimulation is necessary for the activation of T cell receptor-γδ+ T lymphocytes. J Immunol. 1993;151:6043-50. (Costim)
6. Conrad ML, Davis WC, Koop BF. TCR and CD3 antibody cross-reactivity in 44 species. Cytometry. 2007;71A:925-33. (FC, Woodland Caribou Reactivity)
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