Hamster Anti-Mouse KLRG1-UNLB

Cat. No.
1807-01  
Size
0.5 mg  
Price (USD)
$212.00 
Clone 2F1
Isotype Hamster (Syrian) IgG2
Isotype Control Hamster IgG-UNLB
Immunogen IL-2-activated NK cells (A-LAK cells) from B6 (H-2b) mice
Specificity Mouse/Human KLRG1
Alternalte Name(s) MAFA, killer cell lectin-like receptor G1, mast cell function-associated-antigen
Description The monoclonal antibody 2F1 reacts with mouse killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA), a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. The 2F1 antibody stains 30-60% of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL). Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-21
Immunoprecipitation – Reported in literature 1,2
Western Blot-Non-Reducing – Reported in literature 2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795366
  • C57BL/6 mouse splenocytes were stained with Hamster Anti-Mouse KLRG1-FITC (SB Cat. 1807-02) and Mouse Anti-Mouse NK1.1-PE (SB Cat. No. 1805-09).

C57BL/6 mouse splenocytes were stained with Hamster Anti-Mouse KLRG1-FITC (SB Cat. 1807-02) and Mouse Anti-Mouse NK1.1-PE (SB Cat. No. 1805-09).
 
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References
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22. SouthernBiotech unpublished data (FC, Human Reactivity)