Hamster Anti-Mouse CD103-Alexa Fluor® 488

Cat. No.
1810-30  
Size
0.1 mg  
Price (USD)
$190.00 
Clone 2E7
Isotype Hamster (Armenian) IgG2
Isotype Control Hamster IgG-Alexa Fluor® 488
Immunogen C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL)
Specificity Mouse CD103
Alternalte Name(s) Integrin αIEL, αE integrin, ITGAE
Description CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis, and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. The 2E7 monoclonal antibody exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 monoclonal antibody M290. 2E7 is reported to have signal-inducing activity in a redirected lysis assay and to costimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro which runs counter to the usual inhibition of CTL by anti-integrins. This costimulatory activity is also shared by the monoclonal antibody M290. The property of costimulation is not unique to the mouse since the combination of anti-human αIEL plus anti-TCR will produce similar proliferation in human cells with the exception that CD8+ cells are not stimulated.
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 2
Immunoprecipitation – Reported in literature 2
Immunohistochemistry-Frozen Sections – Reported in literature 2
Activation – Reported in literature 3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795382
  • C57BL/6 mouse splenocytes were stained with Hamster Anti-Mouse CD103-AF488 (SB Cat. No. 1810-30) and Rat Anti-Mouse CD8α-PE (SB Cat. No. 1550-09).

C57BL/6 mouse splenocytes were stained with Hamster Anti-Mouse CD103-AF488 (SB Cat. No. 1810-30) and Rat Anti-Mouse CD8α-PE (SB Cat. No. 1550-09).
 
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References
1. Goodman T, Lefrancois L. Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function. J Exp Med. 1989;170:1569-81. (Immunogen)
2. Lefrançois L, Barrett TA, Havran WL, Puddington L. Developmental expression of the αIELβ7 integrin on T cell receptor γδ and T cell receptor αβ T cells. Eur J Immunol. 1994;24:635-40. (IP, IHC-FS, FC)
3. Müller S, Jungo M, Aichele P, Mueller C. CD5- CD8αβ intestinal intraepithelial lymphocytes (IEL) are induced to express CD5 upon antigen-specific activation: CD5- and CD5+ CD8αβ IEL do not represent separate T cell lineages. Eur J Immunol. 1997;27:1756-61. (Activ)
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