Rat Anti-Mouse Ly-6G/Ly-6C-PE

Cat. No.
1900-09  
Size
0.1 mg  
Price (USD)
$165.00 
Clone RB6-8C5
Isotype Rat IgG2bκ
Isotype Control Rat IgG2b-PE
Specificity Mouse Ly-6G and Ly-6C
Alternalte Name(s) Gr-1
Description Ly-6G/Ly-6C, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow. In the periphery, Ly-6G/Ly-6C is expressed predominantly on neutrophils.
Format/Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,11,12
Immunohistochemistry-Frozen Sections – Reported in literature 7-10
Immunoprecipitation – Reported in literature 1
Complement Mediated Cell Depletion – Reported in literature 2
Depletion – Reported in literature 3-6
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795465
  • C57BL/6 mouse bone marrow cells were stained with Rat Anti-Mouse Ly-6G/Ly-6C-PE (SB Cat. No. 1900-09) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).
  • Frozen thermally burned mouse tissue section was stained with Rat Anti-Mouse Ly-6G/Ly-6C-PE (SB Cat. No. 1900-09) and mounted with DAPI containing medium.

    Image from Everett J, Turner K, Cai Q, Gordon V, Whiteley M, Rumbaugh K. Arginine is a critical substrate for the pathogenesis of Pseudomonas aeruginosa in burn wound infections. MBio. 2017;8:e02160-16. Figure 1(c)
  • Frozen thermally burned mouse tissue section was stained with Rat Anti-Mouse CD11b-FITC (SB Cat. No. 1560-02) and Rat Anti-Mouse Ly-6G/Ly-6C-PE (SB Cat. No. 1900-09) followed by mounting with DAPI containing medium.

    Image from Everett J, Turner K, Cai Q, Gordon V, Whiteley M, Rumbaugh K. Arginine is a critical substrate for the pathogenesis of Pseudomonas aeruginosa in burn wound infections. MBio. 2017;8:e02160-16. Figure S1

C57BL/6 mouse bone marrow cells were stained with Rat Anti-Mouse Ly-6G/Ly-6C-PE (SB Cat. No. 1900-09) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).
 

Frozen thermally burned mouse tissue section was stained with Rat Anti-Mouse Ly-6G/Ly-6C-PE (SB Cat. No. 1900-09) and mounted with DAPI containing medium.

Image from Everett J, Turner K, Cai Q, Gordon V, Whiteley M, Rumbaugh K. Arginine is a critical substrate for the pathogenesis of Pseudomonas aeruginosa in burn wound infections. MBio. 2017;8:e02160-16. Figure 1(c)
 

Frozen thermally burned mouse tissue section was stained with Rat Anti-Mouse CD11b-FITC (SB Cat. No. 1560-02) and Rat Anti-Mouse Ly-6G/Ly-6C-PE (SB Cat. No. 1900-09) followed by mounting with DAPI containing medium.

Image from Everett J, Turner K, Cai Q, Gordon V, Whiteley M, Rumbaugh K. Arginine is a critical substrate for the pathogenesis of Pseudomonas aeruginosa in burn wound infections. MBio. 2017;8:e02160-16. Figure S1
 
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References
1. Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993;151:2399-408. (FC, IP)
2. Brummer E, Sugar AM, Stevens DA. Immunological activation of polymorphonuclear neutrophils for fungal killing: studies with murine cells and blastomyces dermatitidis in vitro. J Leukoc Biol. 1984;36:505-20. (CMDC)
3. Franco JL, Ghosh P, Wiltrout RH, Carter CR, Zea AH, Momozaki N, et al. Partial degradation of T-cell signal transduction molecules by contaminating granulocytes during protein extraction of splenic T cells from tumor-bearing mice. Cancer Res. 1995;55:3840-6. (Depletion)
4. Conlan JW, North RJ. Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody. J Exp Med. 1994;179:259-68. (Depletion)
5. Tumpey TM, Chen SH, Oakes JE. Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea. J Virol. 1996;70:898-904. (Depletion)
6. Gong Y, Koh D. Neutrophils promote inflammatory angiogenesis via release of preformed VEGF in an in vivo corneal model. Cell Tissue Res. 2010;339:437-48. (Depletion)
7. Okuma T, Terasaki Y, Kaikita K, Kobayashi H, Kuziel WA, Kawasuji M, et al. C-C chemokine receptor 2 (CCR2) deficiency improves bleomycin-induced pulmonary fibrosis by attenuation of both macrophage infiltration and production of macrophage-derived matrix metalloproteinases. J Pathol. 2004;204:594-604. (IHC-FS)
8. Hasegawa T, Iwanaga K, Hultquist DE, Liao H, Visovatti SH, Pinsky DJ. Suppression of nitrosative and oxidative stress to reduce cardiac allograft vasculopathy. Am J Physiol Heart Circ Physiol. 2009;296:H1007-16. (IHC-FS)
9. Miyazaki Y, Kaikita K, Endo M, Horio E, Miura M, Tsujita K, et al. C/EBP homologous protein deficiency attenuates myocardial reperfusion injury by inhibiting myocardial apoptosis and inflammation. Arterioscler Thromb Vasc Biol. 2011;31:1124-32. (IHC-FS)
10. Hasegawa T, Okada K, Okita Y, Pinsky DJ. Antioxidant properties of pioglitazone limit nicotinamide adenine dinucleotide phosphate hydrogen oxidase and augment superoxide dismutase activity in cardiac allotransplantation. J Heart Lung Transplant. 2011;30:1186-96. (IHC-FS)
11. Scarff KL, Ung KS, Nandurkar H, Crack PJ, Bird CH, Bird PI. Targeted disruption of SPI3/Serpinb6 does not result in developmental or growth defects, leukocyte dysfunction, or susceptibility to stroke. Mol Cell Biol. 2004;24:4075-82. (FC)
12. De Cesare M, Calcaterra C, Pratesi G, Gatti L, Zunino F, Mènard S, et al. Eradication of ovarian tumor xenografts by locoregional administration of targeted immunotherapy. Clin Cancer Res. 2008;14:5512-8. (FC)