Mouse Anti-Rat IgG2b-Alexa Fluor® 488

Cat. No.
3070-30  
Size
0.1 mg  
Price (USD)
$250.00 
Clone 2B10A8
Isotype Mouse (BALB/c) IgG2bκ
Immunogen Rat IgG2b hybridoma
Specificity Rat IgG2b
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-4
ELISA – Quality tested 5,6
FLISA – Quality tested
Immunohistochemistry-Frozen Sections 7
Western Blot 8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795887
  • FLISA plate was coated with purified rat IgG1, IgG2a, IgG2b, IgG2c, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG2b-AF488 (SB Cat. No. 3070-30).
  • C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Mouse Anti-Rat IgG2b-AF488 (SB Cat. No. 3070-30).

FLISA plate was coated with purified rat IgG1, IgG2a, IgG2b, IgG2c, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG2b-AF488 (SB Cat. No. 3070-30).
 

C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Mouse Anti-Rat IgG2b-AF488 (SB Cat. No. 3070-30).
 
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References
1. Piaggio E, Mars LT, Cassan C, Cabarrocas J, Hofstätter M, Desbois S, et al. Multimerized T cell epitopes protect from experimental autoimmune diabetes by inducing dominant tolerance. Proc Natl Acad Sci USA. 2007;104:9393-8. (FC)
2. Vanden Bush TJ, Buchta CM, Claudio J, Bishop GA. Cutting Edge: Importance of IL-6 and cooperation between innate and adaptive immune receptors in cellular vaccination with B lymphocytes. J Immunol. 2009;183:4833-7. (FC)
3. Troeger C, Perahud I, Moser S, Holzgreve W. Transplacental traffic after in utero mesenchymal stem cell transplantation. Stem Cells Dev. 2010;19:1385-92. (FC)
4. Ge W, Jiang J, Liu W, Lian D, Saito A, Garcia B, et al. Regulatory T cells are critical to tolerance induction in presensitized mouse transplant recipients through targeting memory T cells. Am J Transplant. 2010;10:1760-73. (FC)
5. Du F, Lü L, Fu Q, Dai M, Teng J, Fan W, et al. T-614, a novel immunomodulator, attenuates joint inflammation and articular damage in collagen-induced arthritis. Arthritis Res Ther. 2008;10:R136. (ELISA)
6. Pastor M, Esquisabel A, Marquínez I, Talavera A, Pedraz JL. Cellulose acetate phthalate microparticles containing Vibrio cholerae: steps toward an oral cholera vaccine. J Drug Target. 2014;22:478-87. (ELISA)
7. SouthernBiotech published data
8. SouthernBiotech unpublished data
Alexa Fluor® is a registered trademark of Life Technologies Corporation