Mouse Anti-Chicken CD45-BIOT

Cat. No.
8270-08  
Size
0.5 mg  
Price (USD)
$305.00 
Clone LT40
Isotype Mouse (BALB/c) IgMκ
Isotype Control Mouse IgM-BIOT
Immunogen Chicken bursal lymphocytes
Specificity Chicken CD45
Description Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45. Levels of CD45 expression detected by the monoclonal antibody LT40 in the avian thymus are heterogeneous with approximately 90% of thymocytes expressing fourfold higher levels of surface CD45 (CD45hi) than do the remaining 10% (CD45low) of thymocytes. The CD45low population contains exclusively thymocytes with the CD3-CD4-CD8low phenotype characteristic of the immediate precursors to the CD3-CD4+CD8+ thymic population which are CD45hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4-CD8low to CD4+CD8+ and before the expression of CD3. The ability of the monoclonal antibody LT40 to discriminate this CD45low subpopulation provides a suitable marker for the CD3-CD4-CD8low immediate precursors to the CD3-CD4+CD8+ thymocytes.
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,6-18
Immunohistochemistry-Frozen Sections – Reported in literature 2
Immunohistochemistry-Paraffin Sections – Reported in literature 3
Immunohistochemistry-Whole Mount – Reported in literature 4
Immunocytochemistry – Reported in literature 19
Immunoprecipitation – Reported in literature 1
Separation – Reported in literature 5
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796473
  • Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD45-BIOT (SB Cat. No. 8270-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD45-BIOT (SB Cat. No. 8270-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
 
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References
1. Paramithiotis E, Tkalec L, Ratcliffe MJ. High levels of CD45 are coordinately expressed with CD4 and CD8 on avian thymocytes. J Immunol. 1991;147:3710-7. (Immunogen, FC, IP)
2. Corbel C. Expression of αVβ3 integrin in the chick embryo aortic endothelium. Int J Dev Biol. 2002;46:827-30. (IHC-FS)
3. Teixeira AR, Gomes C, Nitz N, Sousa AO, Alves RM, Guimaro MC, et al. Trypanosoma cruzi in the chicken model: Chagas-like heart disease in the absence of parasitism. PLoS Negl Trop Dis. 2011;5(3):e1000. (IHC-PS)
4. Bhave SA, Oesterle EC, Coltrera MD. Macrophage and microglia-like cells in the avian inner ear. J Comp Neurol. 1998;398:241-56. (IHC)
5. del Cacho E, Gallego M, Lee SH, Lillehoj HS, Quilez J, Lillehoj EP, et al. Induction of protective immunity against Eimeria tenella infection using antigen-loaded dendritic cells (DC) and DC-derived exosomes. Vaccine. 2011;29:3818-25. (Sep)
6. Islam AF, Wong CW, Walkden-Brown SW, Colditz IG, Arzey KE, Groves PJ. Immunosuppressive effects of Marek's disease virus (MDV) and herpesvirus of turkeys (HVT) in broiler chickens and the protective effect of HVT vaccination against MDV challenge. Avian Pathol. 2002;31:449-61. (FC)
7. Schienda J, Engleka KA, Jun S, Hansen MS, Epstein JA, Tabin CJ. Somitic origin of limb muscle satellite and side population cells. Proc Natl Acad Sci USA. 2006;103:945-50. (FC)
8. Buitenhuis AJ, Kjaer JB, Labouriau R, Juul-Madsen HR. Altered circulating levels of serotonin and immunological changes in laying hens divergently selected for feather pecking behavior. Poult Sci. 2006;85:1722-8. (FC)
9. Nera K, Alinikula J, Terho P, Narvi E, Törnquist K, Kurosaki T, et al. Ikaros has a crucial role in regulation of B cell receptor signaling. Eur J Immunol. 2006;36:516-25. (FC)
10. Dalgaard T, Boving MK, Handberg K, Jensen KH, Norup LR, Juul-Madsen HR. MHC expression on spleen lymphocyte subsets in genetically resistant and susceptible chickens infected with Marek's disease virus. Viral Immunol. 2009;22:321-7. (FC)
11. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)
12. Alinikula J, Kohonen P, Nera K, Lassila O. Concerted action of Helios and Ikaros controls the expression of the inositol 5-phosphatase SHIP. Eur J Immunol. 2010;40:2599-607. (FC)
13. Alinikula J, Nera K, Junttila S, Lassila O. Alternate pathways for Bcl6-mediated regulation of B cell to plasma cell differentiation. Eur J Immunol. 2011;41:2404-13. (FC)
14. Juul-Madsen HR, Norup LR, Jørgensen PH, Handberg KJ, Wattrang E, Dalgaard TS. Crosstalk between innate and adaptive immune responses to infectious bronchitis virus after vaccination and challenge of chickens varying in serum mannose-binding lectin concentrations. Vaccine. 2011;29:9499-507. (FC)
15. Fair JM, Nemeth NM, Taylor-McCabe KJ, Shou Y, Marrone BL. Clinical and acquired immunologic responses to West Nile virus infection of domestic chickens (Gallus gallus domesticus). Poult Sci. 2011;90:328-36. (FC)
16. Matulova M, Stepanova H, Sisak F, Havlickova H, Faldynova M, Kyrova K, et al. Cytokine signaling in splenic leukocytes from vaccinated and non-vaccinated chickens after intravenous infection with Salmonella enteritidis. PLoS One. 2012;7(2):e32346. (FC)
17. Revajová V, Slaminková Z, Grešáková L, Levkut M. Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol. Acta Vet. Brno. 2013;82:337-42. (FC)
18. Andersen JP, Norup LR, Dalgaard TS, Rothwell L, Kaiser P, Permin A, et al. No protection in chickens immunized by the oral or intra-muscular immunization route with Ascaridia galli soluble antigen. Avian Pathol. 2013;42:276-82. (FC)
19. Sonoda Y, Abdel Mageed AM, Isobe N, Yoshimura Y. Induction of avian β-defensins by CpG oligodeoxynucleotides and proinflammatory cytokines in hen vaginal cells in vitro. Reproduction. 2013;145:621-31. (ICC)