Mouse Anti-Chicken Ia-PE

Cat. No.
8290-09  
Size
0.1 mg  
Price (USD)
$215.00 
Clone CIa
Isotype Mouse (BALB/c) IgMκ
Isotype Control Mouse IgM-PE
Immunogen Splenic leukocytes from an outbred nine-week-old chicken
Specificity Chicken/Quail/Dove/Pigeon/Duck Ia
Alternalte Name(s) MHC Class II
Description The chicken Ia antigen is expressed on B cells, a subpopulation of the monocyte/macrophage lineage of cells, and mitogen-activated T cells. The molecule recognized by this antibody is similar in structure to the murine Ia antigens and human DR antigens.
Format/Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,7,9,10
Immunohistochemistry-Frozen Sections – Reported in literature 2-6
Immunocytochemistry – Reported in literature 1,7,8
Immunoprecipitation – Reported in literature 1
Blocking – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796485
  • Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken Ia-PE (SB Cat. No. 8290-09) and Mouse Anti-Chicken CD3-FITC (SB Cat. No. 8200-02).

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken Ia-PE (SB Cat. No. 8290-09) and Mouse Anti-Chicken CD3-FITC (SB Cat. No. 8200-02).
 
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References
1. Ewert DL, Munchus MS, Chen CH, Cooper MD. Analysis of structural properties and cellular distribution of avian Ia antigen by using monoclonal antibody to monomorphic determinants. J Immunol. 1984;132:2524-30. (Immunogen, FC, ICC, IP, Block, Quail, Dove, Pigeon, & Duck Reactivity)
2. Wang X, Efr GF. Apoptosis in feathers of Smyth line chickens with autoimmune vitiligo. J Autoimmun. 2004;22:21-30. (IHC-FS)
3. O'Halloran EK, Oesterle EC. Characterization of leukocyte subtypes in chicken inner ear sensory epithelia. J Comp Neurol. 2004;475:340-60. (IHC-FS)
4. Das SC, Nagasaka N, Yoshimura Y. Changes in the localization of antigen presenting cells and T cells in the utero-vaginal junction after repeated artificial insemination in laying hens. J Reprod Dev. 2005;51:683-7. (IHC-FS)
5. Subedi K, Yoshimura Y. Expression of MHC class I and II in growing ovarian follicles of young and old laying hens, Gallus domesticus. J Poult Sci. 2005;42:101-9. (IHC-FS)
6. Yoshimura Y, Fukui T, Nishibori M, Isobe N. Effects of age and gonadal steroids on the localization of antigen presenting cells in the epididymis of the male chicken, Gallus domesticus. J Reprod Dev. 2006;52:363-71. (IHC-FS)
7. Bakri Y, Sarrazin S, Mayer UP, Tillmanns S, Nerlov C, Boned A, et al. Balance of MafB and PU.1 specifies alternative macrophage or dendritic cell fate. Blood. 2005;105:2707-16. (FC, ICC)
8. Niikura M, Kim T, Hunt HD, Burnside J, Morgan RW, Dodgson JB, et al. Marek's disease virus up-regulates major histocompatibility complex class II cell surface expression in infected cells. Virology. 2007;359:212-9. (ICC)
9. Rautenschlein S, Subramanian A, Sharma JM. Bioactivities of a tumour necrosis-like factor released by chicken macrophages. Dev Comp Immunol. 1999;23:629-40. (FC)
10. Chrząstek K, Piasecki T, Wieliczko A. Impact of CpG oligodeoxynucleotide stimulation on percentage of T and B cells in chicken. Pol J Vet Sci. 2013;16:551-4. (FC)