Mouse Anti-Human IgG Fc-Alexa Fluor® 555

Cat. No.
9042-32  
Size
0.1 mg  
Price (USD)
$250.00 
Clone H2
Isotype Mouse IgG2bk
Specificity Human/Rhesus/Cynomolgus/Chimpanzee/African Green Monkey/Mangabey/Pigtail IgG Fc
Format/Conjugate AF555 (Alexa Fluor® 555)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested 1
FLISA – Quality tested
Flow Cytometry – Quality tested 4
ELISpot – Reported in literature 2
Western Blot – Reported in literature 3
Multiplex – Reported in literature 5-9
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796616
  • FLISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG Fc-AF555 (SB Cat. No. 9042-32).

FLISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG Fc-AF555 (SB Cat. No. 9042-32).
 
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References
1. Vlasak J, Bussat MC, Wang S, Wagner-Rousset E, Schaefer M, Klinguer-Hamour C, et al. Identification and characterization of asparagine deamidation in the light chain CDR1 of a humanized IgG1 antibody. Anal Biochem. 2009;392:145-54. (ELISA)
2. Mahal SP, Baker CA, Demczyk CA, Smith EW, Julius C, Weissmann C. Prion strain discrimination in cell culture: the cell panel assay. Proc Natl Acad Sci USA. 2007;104:20908-13. (ELISPOT)
3. Goodman RE, Panda R, Ariyarathna H. Evaluation of endogenous allergens for the safety evaluation of genetically engineered food crops: review of potential risks, test methods, examples and relevance. J Agric Food Chem. 2013;61:8317-32. (WB)
4. He B, Xu W, Santini PA, Polydorides AD, Chiu A, Estrella J, et al. Intestinal bacteria trigger T cell-independent immunoglobulin A2 class switching by inducing epithelial-cell secretion of the cytokine APRIL. Immunity. 2007;26:812-26. (FC)
5. Moss DM, Priest JW, Boyd A, Weinkopff T, Kucerova Z, Beach MJ, et al. Multiplex bead assay for serum samples from children in Haiti enrolled in a drug study for the treatment of lymphatic filariasis. Am J Trop Med Hyg. 2011;85:229-37. (Multiplex)
6. Priest JW, Moss DM, Won K, Todd CW, Henderson L, Jones CC, et al. Multiplex assay detection of immunoglobulin G antibodies that recognize Babesia microti antigens. Clin Vaccine Immunol. 2012;19:1539-48. (Multiplex)
7. Goodhew EB, Priest JW, Moss DM, Zhong G, Munoz B, Mkocha H, et al. CT694 and pgp3 as serological tools for monitoring trachoma programs. PLoS Negl Trop Dis. 2012;6(11):e1873. (Multiplex)
8. Yang G, Holl TM, Liu Y, Li Y, Lu X, Nicely NI, et al. Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies. J Exp Med. 2013;210:241-56. (Multiplex)
9. Goodhew EB, Morgan SM, Switzer AJ, Munoz B, Dize L, Gaydos C, et al. Longitudinal analysis of antibody responses to trachoma antigens before and after mass drug administration. BMC Infect Dis. 2014;14:216. (Multiplex)
10. Nonhuman Primate Reagent Resource (Rhesus, Cynomolgus, Chimpanzee, African Green Monkey, Mangabey, & Pigtail Reactivity)
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