Mouse Anti-Human CD4-Alexa Fluor® 700

Cat. No.
9522-27  
Size
100 tests  
Price (USD)
$220.00 
Clone RFT4
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-Alexa Fluor® 700
Immunogen Thymocytes and E-rosetted lymphocytes
Specificity Human CD4
Alternalte Name(s) T4, Leu-3
Description CD4 is a 55 kDa type I transmembrane glycoprotein and a member of the Ig-SF of cell surface receptors. It is expressed predominantly on the “helper/inducer” subpopulation of mature T lymphocytes and on monocytes and macrophages. Domains 1 and 2 bind to MHC Class II antigens, while domains 3 and 4 may be involved in cis interactions with the CD3/TCR complex. CD4 functions as an accessory molecule in the recognition of foreign antigens in association with MHC class II antigens by T cells.
Format/Conjugate AF700 (Alexa Fluor® 700)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,7-9
Immunohistochemistry-Frozen Sections – Reported in literature 3
Immunocytochemistry – Reported in literature 4,5
Western Blot – Reported in literature 2
Separation – Reported in literature 6
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796859
  • Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4-PE/CY5.5 (SB Cat. No. 9522-16) and Mouse Anti-Human CD8-PE (SB Cat. No. 9536-09).

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4-PE/CY5.5 (SB Cat. No. 9522-16) and Mouse Anti-Human CD8-PE (SB Cat. No. 9536-09).
 
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References
1. Sattentau QJ, Arthos J, Deen K, Hanna N, Healey D, Beverley PC, et al. Structural analysis of the human immunodeficiency virus-binding domain of CD4. Epitope mapping with site-directed mutants and anti-idiotypes. J Exp Med. 1989;170:1319-34. (FC)
2. Eugene HS, Pierce-Paul BR, Craigo JK, Ross TM. Rhesus macaques vaccinated with consensus envelopes elicit partially protective immune responses against SHIV SF162p4 challenge. Virol J. 2013;10:102. (WB)
3. Hufert FT, van Lunzen J, Janossy G, Bertram S, Schmitz J, Haller O, et al. Germinal centre CD4+ T cells are an important site of HIV replication in vivo. AIDS. 1997;11:849-57. (IHC-FS)
4. Poulter LW, Norris A, Powers C, Condez A, Burnes H, Schmekel B, et al. T cell dominated inflammatory reactions in the bronchioles of asymptomatic asthmatics are also present in the nasal mucosa. Postgrad Med J. 1991;67:747-53. (ICC)
5. Burgess R, Hyde K, Maguire PJ, Kelsey PR, Yin JA, Geary CG. Two-colour immunoenzymatic technique using sequential staining by APAAP to evaluate two cell antigens. J Clin Pathol. 1992;45:206-9. (ICC)
6. D'Acquisto F, Paschalidis N, Raza K, Buckley CD, Flower RJ, Perretti M. Glucocorticoid treatment inhibits annexin-1 expression in rheumatoid arthritis CD4+ T cells. Rheumatology. 2008;47:636-9. (Sep)
7. Daubenberger CA, Salomon M, Vecino W, Hübner B, Troll H, Rodriques R, et al. Functional and structural similarity of Vγ9V δ2 T cells in humans and Aotus monkeys, a primate infection model for Plasmodium falciparum malaria. J Immunol. 2001;167:6421-30. (FC)
8. Zariffard MR, Saifuddin M, Finnegan A, Spear GT. HSV type 2 infection increases HIV DNA detection in vaginal tissue of mice expressing human CD4 and CCR5. AIDS Res Hum Retroviruses. 2009;25:1157-64. (FC)
9. Roetynck S, Olotu A, Simam J, Marsh K, Stockinger B, Urban B, et al. Phenotypic and functional profiling of CD4 T cell compartment in distinct populations of healthy adults with different antigenic exposure. PLoS One. 2013;8(1):e55195. (FC)
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