Mouse Anti-Human CD8-APC/CY5.5

Cat. No.
9536-18  
Size
100 tests  
Price (USD)
$270.00 
Clone RFT8
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-APC/CY5.5
Immunogen Thymocytes and E-rosetted lymphocytes
Specificity Human CD8
Alternalte Name(s) Leu-2, Lyt2, Lyt3, Tp32, T8
Description CD8 is a 33 kDa transmembrane glycoprotein expressed as either a CD8αα homodimer or CD8αβ heterodimer. It is expressed on the “cytotoxic/suppressor” subpopulation of peripheral T cells. CD8 functions primarily as a coreceptor with MHC class I-restricted TCR’s in antigen recognition.
Format/Conjugate APC/CY5.5 (Allophycocyanin/Cyanine 5.5)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,9
Immunohistochemistry-Frozen Sections – Reported in literature 2-5
Immunohistochemistry-Paraffin Sections – Reported in literature 6
Immunocytochemistry – Reported in literature 7
Depletion – Reported in literature 8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796891
  • Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD8-APC/CY5.5 (SB Cat. No. 9536-18) and Mouse Anti-Human CD4-FITC (SB Cat. No. 9522-02).

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD8-APC/CY5.5 (SB Cat. No. 9536-18) and Mouse Anti-Human CD4-FITC (SB Cat. No. 9522-02).
 
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References
1. Bofill M, Janossy G, Lee CA, MacDonald-Burns D, Phillips AN, Sabin C, et al. Laboratory control values for CD4 and CD8 T lymphocytes. Implications for HIV-1 diagnosis. Clin Exp Immunol. 1992;88:243-52. (Immunogen, FC)
2. Bofill M, Gombert W, Borthwick NJ, Akbar AN, McLaughlin JE, Lee CA, et al. Presence of CD3+CD8+Bcl-2low lymphocytes undergoing apoptosis and activated macrophages in lymph nodes of HIV-1+ patients. Am J Pathol. 1995;146:1542-55. (IHC-FS)
3. Gay FW, Drye TJ, Dick GW, Esiri MM. The application of multifactorial cluster analysis in the staging of plaques in early multiple sclerosis. Identification and characterization of the primary demyelinating lesion. Brain. 1997;120:1461-83. (IHC-FS)
4. Hufert FT, van Lunzen J, Janossy G, Bertram S, Schmitz J, Haller O, et al. Germinal centre CD4+ T cells are an important site of HIV replication in vivo. AIDS. 1997;11:849-57. (IHC-FS)
5. Howie D, Spencer J, DeLord D, Pitzalis C, Wathen NC, Dogan A, et al. Extrathymic T cell differentiation in the human intestine early in life. J Immunol. 1998;161:5862-72. (IHC-FS)
6. Cruickshank SM, Southgate J, Wyatt JI, Selby PJ, Trejdosiewicz LK. Expression of CD44 on bile ducts in primary sclerosing cholangitis and primary biliary cirrhosis. J Clin Pathol. 1999;52:730-4. (IHC-PS)
7. Burgess R, Hyde K, Maguire PJ, Kelsey PR, Yin JA, Geary CG. Two-colour immunoenzymatic technique using sequential staining by APAAP to evaluate two cell antigens. J Clin Pathol. 1992;45:206-9. (ICC)
8. Wimperis JZ, Gottlieb D, Duncombe AS, Heslop HE, Prentice HG, Brenner MK. Requirements for the adoptive transfer of antibody responses to a priming antigen in man. J Immunol. 1990;144:541-7. (Depletion)
9. Hunter PJ, Nistala K, Jina N, Eddaoudi A, Thomson W, Hubank M, et al. Biologic predictors of extension of oligoarticular juvenile idiopathic arthritis as determined from synovial fluid cellular composition and gene expression. Arthritis Rheum. 2010;62:896-907. (FC)
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