Mouse Anti-Human CD14-APC/CY5.5

Cat. No.
9561-18  
Size
100 tests  
Price (USD)
$285.00 
Clone 61D3
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-APC/CY5.5
Immunogen Human peripheral monocytes
Specificity Human/Cynomolgus/Canine/Hooded Seal CD14
Alternalte Name(s) LPS-R, LPS receptor, gp55
Description CD14 is a 55 kDa glycosphosphatidylinositol (GPI)-anchored single chain glycoprotein. It is expressed on monocytes, macrophages, dendritic cells, and Langerhans cells. It is also weakly expressed on neutrophils. CD14 appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.
Format/Conjugate APC/CY5.5 (Allophycocyanin/Cyanine 5.5)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 3,6-8
Immunohistochemistry-Frozen Sections – Reported in literature 4
Immunocytochemistry – Reported in literature 3,5
Western Blot – Reported in literature 3
ELISA – Reported in literature 3
Cytoxicity – Reported in literature 1
Blocking – Reported in literature 2,3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796943
  • Human peripheral blood monocytes were stained with Mouse Anti-Human CD14-PE/CY5.5 (SB Cat. No. 9561-16).

Human peripheral blood monocytes were stained with Mouse Anti-Human CD14-PE/CY5.5 (SB Cat. No. 9561-16).
 
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References
1. Ugolini V, Nunez G, Smith RG, Stastny P, Capra JD. Initial characterization of monoclonal antibodies against human monocytes. Proc Natl Acad Sci USA. 1980;77:6764-8. (Immunogen, Cyto)
2. Flora PK, Gregory CD. Recognition of apoptotic cells by human macrophages: inhibition by a monocyte/macrophage-specific monoclonal antibody. Eur J Immunol. 1994;24:2625-32. (Block)
3. Devitt A, Moffatt OD, Raykundalia C, Capra JD, Simmons DL, Gregory CD. Human CD14 mediates recognition and phagocytosis of apoptotic cells. Nature. 1998;392:505-9. (Block, WB, FC, ELISA, ICC)
4. Beckstead JH, Wood GS, Fletcher V. Evidence for the origin of Kaposi's sarcoma from lymphatic endothelium. Am J Pathol. 1985;119:294-300. (IHC-FS)
5. Larsen AK, Nymo IH, Boysen P, Tryland M, Godfroid J. Entry and elimination of marine mammal Brucella spp. by hooded seal (Cystophora cristata) alveolar macrophages in vitro. PLoS One. 2013;8(7):e70186. (ICC, Hooded Seal Reactivity)
6. Pilling D, Vakil V, Gomer RH. Improved serum-free culture conditions for the differentiation of human and murine fibrocytes. J Immunol Methods. 2009;351:62-70. (FC)
7. Carter AS, Cerundolo L, Koo DD, Rust NA, Morris PJ, Fuggle SV. Cross-species reactivity of a panel of antibodies with monkey and porcine tissue. Xenotransplantation. 1999;6:123-30. (FC, Cynomolgus Reactivity)
8. de Amorim IF, da Silva SM, Figueiredo MM, Moura EP, de Castro RS, de Souza Lima TK, et al. Toll receptors type-2 and CR3 expression of canine monocytes and its correlation with immunohistochemistry and xenodiagnosis in visceral leishmaniasis. PLoS One. 2011;6(11):e27679. (FC, Canine Reactivity)
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