Mouse Anti-Human CD45-PE/CY5.5

Cat. No.
9625-16  
Size
100 tests  
Price (USD)
$265.00 
Clone F10-89-4
Isotype Mouse IgG2aκ
Isotype Control Mouse IgG2a-PE/CY5.5
Immunogen Purified T cells from human lymph nodes
Specificity Human/Horse CD45
Alternalte Name(s) LCA, T200, Ly5
Description CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220 kDa) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation.
Format/Conjugate PE/CY5.5 (R-phycoerythrin/Cyanine 5.5)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,5,9-11
Immunohistochemistry-Frozen Sections – Reported in literature 1-3
Immunocytochemistry – Reported in literature 4,5
Immunoprecipitation – Reported in literature 6,7
Western Blot – Reported in literature 7,8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2797031
  • Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45-PE/CY5.5 (SB Cat. No. 9625-16).

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45-PE/CY5.5 (SB Cat. No. 9625-16).
 
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References
1. Dalchau R, Kirkley J, Fabre JW. Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat. Eur J Immunol. 1980;10:737-44. (Immunogen, FC, IHC-FS)
2. Mason DY, Cordell JL, Abdulaziz Z, Naiem M, Bordenave G. Preparation of peroxidase: antiperoxidase (PAP) complexes for immunohistological labeling of monoclonal antibodies. J Histochem Cytochem. 1982;30:1114-22. (IHC-FS)
3. Salter DM, Krajewski AS, Dewar AE. Immunophenotype analysis of malignant histiocytosis of the intestine. J Clin Pathol. 1986;39:8-15. (IHC-FS)
4. Toscano MA, Bianco GA, Ilarregui JM, Croci DO, Correale J, Hernandez JD, et al. Differential glycosylation of TH1, TH2 and TH-17 effector cells selectively regulates susceptibility to cell death. Nat Immunol. 2007;8:825-34. (ICC)
5. De Schauwer C, Piepers S, Van de Walle GR, Demeyere K, Hoogewijs MK, Govaere JL, et al. In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry. Cytometry A. 2012;81:312-23. (FC, ICC, Horse Reactivity)
6. Chen I, Chen H, Demetriou M. Lateral compartmentalization of T cell receptor versus CD45 by galectin-N-glycan binding and microfilaments coordinate basal and activation signaling. J Biol Chem. 2007;282:35361-72. (IP)
7. Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I. Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J Immunol. 2004;172:4298-307. (IP, WB)
8. Stillman BN, Hsu DK, Pang M, Brewer CF, Johnson P, Liu F, et al. Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death. J Immunol. 2006;176:778-89. (WB)
9. Young AJ, Holzgreve W, Dudler L, Schoeberlein A, Surbeck DV. Engraftment of human cord blood-derived stem cells in preimmune ovine fetuses after ultrasound-guided in utero transplantation. Am J Obstet Gynecol. 2003;189:698-701. (FC)
10. Mitchell P, Lee F, Hall C, Rigopoulos A, Smyth FE, Hekman A, et al. Targeting primary human Ph+ B-cell precursor leukemia-engrafted SCID mice using radiolabeled anti-CD19 monoclonal antibodies. J Nucl Med. 2003;44:1105-12. (FC)
11. Røsland GV, Svendsen A, Torsvik A, Sobala E, McCormack E, Immervoll H, et al. Long-term cultures of bone marrow-derived
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