Mouse Anti-Human CD107a-BIOT

Cat. No.
9835-08  
Size
100 tests  
Price (USD)
$178.00 
Clone H4A3
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-BIOT
Immunogen Human adherent peripheral blood cells
Specificity Human/Rhesus/African Green Monkey/Pigtail Macaque/Sooty Mangabey CD107a
Alternalte Name(s) LAMP-1, lysosome-associated membrane protein 1
Description CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated type I transmembrane protein that constitutes the major sialoglycoproteins on lysosomal membranes. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. CD107a is expressed by activated T cells, macrophages, dendritic cells, activated platelets, tonsillar epithelium, and some tumor cell lines, including U937 and KG1a. It is also a widely expressed intracellular antigen. LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. The upregulation of both CD107a and CD107b on the surface of tumor cell lines has been associated with their enhanced metastatic potential where they may increase adhesion to extracellular matrix and endothelium.
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,12-19,21,22
Immunohistochemistry-Frozen Sections – Reported in literature 2
Immunohistochemistry-Paraffin Sections – Reported in literature 3,4
Immunocytochemistry – Reported in literature 5-10,20
Electron Microscopy – Reported in literature 1
Immunoprecipitation – Reported in literature 1,5,6
Western Blot – Reported in literature 11
Purification – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2797105
  • Human T cell leukemia cell line Jurkat was intracellularly stained with Mouse Anti-Human CD107a-BIOT (SB Cat. No. 9835-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

Human T cell leukemia cell line Jurkat was intracellularly stained with Mouse Anti-Human CD107a-BIOT (SB Cat. No. 9835-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
 
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References
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