Hybridoma Development & Monoclonal Antibody Production Services
SouthernBiotech offers a broad range of services for the development and production of monoclonal antibodies. Our Hybridoma
Development and Monoclonal Antibody Production Services include - immunization, cell fusion, cloning, characterization, and
production of your monoclonal antibody in vivo in BALB/c or immunocompromized (e.g., SCID, Nu/Nu, RAG-/-) mice, or in vitro in
flasks or bioreactors. Additional services include antibody purification and conjugation to fluorochromes and enzymes.
| Available Services for Hybridoma Development |
Cat. No. |
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Pre-Immunization Antigen Processing and Modification
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Homogenization of Antigen in Gels or Nitrocellulose Slices
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SBCS-19
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Conjugation to KLH for Immunization
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SBCS-22K
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Conjugation to BSA for Screening
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SBCS-22B
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Dialysis into Physiological Buffer
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SBCS-22D
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Phase I - Immunization and Evaluation of Antibody Titer
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SBCS-30I
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Immunization of Three (3) BALB/c mice with your supplied and purified antigen.
Includes Four (4) immunizations per our standard protocol and daily care. Test bleeds and ELISA data will be
provided for your evaluation and decision to continue development.
|
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Phase II - Fusion, Plating, and Screening
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SBCS-30II
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Splenocytes of one of the mice are fused with a selected myeloma cell line. All specific antibody producing
colonies are selected by ELISA for expansion. Supernatant from Five (5) of the best hybridoma-containing wells
will be shipped to you for screening. Cryopreservation of up to Five (5) positive clones.
|
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Phase III - Cloning, Expansion, and Cryopreservation
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SBCS-30III
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Subcloning of one positive clone to monoclonality by two rounds of limiting dilution. ELISA screening to detect
positive antibody-secreting subclones. Cryopreservation of Ten (10) vials of the hybridoma cell line and isotyping
of one monoclonal antibody.
|
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Phase IV - Amplification
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SBCS-30IV
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Production and shipment of monoclonal antibody supernatant from one selected clone
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| Available Services for Monoclonal Production |
|
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Subcloning, Expansion, and Screening of Clones
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SBCS-30S
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Cryopreservation - Up to Twenty (20) Clones per Month
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SBCS-30C
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Mycoplasma Testing
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SBCS-29
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Ascites Production in Ten (10) BALB/c Mice
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SBCS-31
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Ascites Production in Twenty (20) BALB/c Mice
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SBCS-32
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Ascites Production in Fifty (50) BALB/c Mice
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SBCS-33
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Ascites Production in One-Hundred (100) BALB/c Mice
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SBCS-34
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Ascites Production in Ten (10) Immunocompromised Mice
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SBCS-35
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Ascites Production in Twenty (20) Immunocompromised Mice
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SBCS-36
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Ascites Production in Fifty (50) Immunocompromised Mice
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SBCS-37
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Ascites Production in One-Hundred (100) Immunocompromised Mice
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SBCS-38
|
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In vitro Production in Cell Culture Flask - Fifty (50) to One-Hundred (100) mL
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SBCS-91
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In vitro Production in Cell Culture Flask - Thirty (30) mL
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SBCS-92
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In vitro Production in Bioreactor
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SBCS-90
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Note – Prior to initiating an antibody development project, the customer must provide SouthernBiotech with a signed
assurance that any customer-supplied material is mycoplasma-free, non-infectious, non-carcinogenic, and has no known
safety concerns to animals or humans.
To initiate a Custom Service Project -
Contact our Technical Services Department at 205.945.1774 or
Toll-free within the US and Canada 800.722.2255
A project manager will be assigned and report to you regularly on an agreed schedule.
Discounts and third party arrangements are not accepted in conjunction with SouthernBiotech Custom Services.
All custom services are accepted and undertaken on a “best efforts” basis only. SouthernBiotech cannot and does not make
any representations or warranties, expressed or implied, including any warranty of merchantability or fitness for any particular
purpose for any material produced or service provided; and, all charges are due and payable as billed regardless of the outcome
of our work