Bovine Type V Collagen-Solution

Cat. No.
1280-02S  
Size
0.25 mg  
Price (USD)
$215.00 
Description Collagen is the main structural protein in the extracellular space and is the most abundant protein in the ECM. Collagens are divided into two classes - fibril (types I, II, III, V) and non-fibril (types IV, VI). Type V collagen is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen mutations are associated with Ehlers-Danlos syndrome. Type V collagen is broadly expressed as a two α1(V) chains and one α2(V) chain heterotrimer but also as a α1(V), α2(V), and α3(V) heterotrimer in pancreatic islets, adipose tissue, and skeletal muscle.
Source Placental villi
Purity > 90% by SDS-PAGE
Purification Controlled and limited pepsin digestion followed by selective salt precipitation
Alternalte Name(s) COL5A1, COL5A2, COL5A3
Buffer Formulation 500 mM acetic acid
Concentration 0.5 mg/mL
Volume 0.5 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications ELISA – Quality tested
SDS-PAGE – Quality tested
Surface Plasmon Resonance – Reported in literature 1
Coating Material for –
Adhesion Studies – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with serially diluted Bovine Type V Collagen (SB Cat. No. 1280-02S). Purified collagen was detected with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01), Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01), Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01), Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01), Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01), and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).

ELISA plate was coated with serially diluted Bovine Type V Collagen (SB Cat. No. 1280-02S). Purified collagen was detected with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01), Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01), Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01), Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01), Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01), and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
 
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References
1. House-Pompeo K, Boles JO, Höök M. Characterization of bacterial adhesin interactions with extracellular matrix components utilizing biosensor technology. Methods. 1994;6:134-42. (SPR, Coat, Adhesion Studies)