Bovine Type VI Collagen-Solution

Cat. No.
1300-02S  
Size
0.25 mg  
Price (USD)
$300.00 
Description Collagen is the main structural protein in the extracellular space and is the most abundant protein in the ECM. Collagens are divided into two classes - fibril (types I, II, III, V) and non-fibril (types IV, VI). Type VI collagen is one of the major components of skeletal muscle and white adipose tissue extracellular matrix and is abundantly expresses by skin fibroblasts. Type VI collagen mutations are associated with Bethlem myopathy and Ullrich congenital muscular dystrophy. Type VI collagen consists of α1(VI), α2(VI), and α3(VI) chains as well the more recently identified α4(VI), α5(VI), and α6(VI) chains with α4(VI) being nonfunctional in humans.
Source Placental villi
Purity > 90% by SDS-PAGE
Purification Controlled and limited pepsin digestion followed by selective salt precipitation
Alternalte Name(s) COL6A1, COL6A2, COL6A3, COL6A4, COL6A5, COL6A6
Buffer Formulation 500 mM acetic acid
Concentration 0.5 mg/mL
Volume 0.5 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications ELISA – Quality tested
SDS-PAGE – Quality tested
Surface Plasmon Resonance – Reported in literature 1
Coating Material for –
Adhesion Studies – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with serially diluted Bovine Type VI Collagen (SB Cat. No. 1300-02S). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

ELISA plate was coated with serially diluted Bovine Type VI Collagen (SB Cat. No. 1300-02S). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
1. House-Pompeo K, Boles JO, Höök M. Characterization of bacterial adhesin interactions with extracellular matrix components utilizing biosensor technology. Methods. 1994;6:134-42. (SPR, Coat, Adhesion Studies)