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SBA Clonotyping System-C57BL/6-AP

Cat. No.
5300-04B

Size
1 kit

Price (USD)
$450.00

Qty	Add to Cart

Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Description	The SBA Clonotyping System-C57BL/6-AP kit is designed for the isotyping of C57BL/6 mouse monoclonal antibodies. It contains 2.5 mg of capture antibody and 1.0 mL of AP conjugated anti-mouse Ig, mouse IgA, mouse IgG₁, mouse IgG_2b, mouse IgG_2c, mouse IgG₃, mouse IgM, mouse κ, mouse λ, and pNPP substrate. The kit may also be utilized for quantitative studies of mouse immunoglobulins in samples such as serum, supernatant, and ascites when used in conjunction with the C57BL/6 Mouse Immunoglobulin Panel (SB Cat. No. 5300-01B).
Kit Components	Goat Anti-Mouse Ig, Human ads-UNLB - 2.5 mg in 1.0 mL Goat Anti-Mouse Ig, Human ads-AP - 1.0 mL Goat Anti-Mouse IgA-AP - 1.0 mL Goat Anti-Mouse IgG₁, Human ads-AP - 1.0 mL Goat Anti-Mouse IgG_2b, Human ads-AP - 1.0 mL Goat Anti-Mouse IgG_2c, Human ads-AP - 1.0 mL Goat Anti-Mouse IgG₃, Human ads-AP - 1.0 mL Goat Anti-Mouse IgM, Human ads-AP - 1.0 mL Goat Anti-Mouse Kappa-AP - 1.0 mL Goat Anti-Mouse Lambda-AP - 1.0 mL pNPP Substrate Tablets - 20 x 5 mg
Buffer Formulation	UNLB antibody - Borate buffered saline, pH 8.2 AP conjugates - 50 mM Tris/1 mM MgCl₂/50% Glycerol containing 0.1% sodium azide, pH 8.0
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	ELISA – Quality tested ^1-4
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2796079

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References

1.	Dubovsky JA, Beckwith KA, Natarajan G, Woyach JA, Jaglowski S, Zhong Y, et al. Ibrutinib is an irreversible molecular inhibitor of ITK driving a Th1-selective pressure in T lymphocytes. Blood. 2013;122:2539-49. (ELISA - Serum)
2.	Hwang I, Park C, Luong T, Harrison KA, Birnbaumer L, Kehrl JH. The loss of Gnai2 and Gnai3 in B cells eliminates B lymphocyte compartments and leads to a hyper-IgM like syndrome. PLoS One. 2013;8(8):e72596. (ELISA - Serum)
3.	Park KS, Bayles I, Szlachta-McGinn A, Paul J, Boiko J, Santos P, et al. Transcription elongation factor ELL2 drives Ig secretory-specific mRNA production and the unfolded protein response. J Immunol. 2014;193:4663-74. (ELISA - Serum)
4.	Hwang I, Park C, Harrison K, Boularan C, Galés C, Kehrl JH. An essential role for RGS protein/Gα_i2 interactions in B lymphocyte-directed cell migration and trafficking. J Immunol. 2015;194:2128-39. (ELISA - Serum)

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