SBA Clonotyping System-C57BL/6-AP

Cat. No.
5300-04B  
Size
1 kit   		Price (USD)
$450.00 
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Technical Bulletin Certificate of
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Description The SBA Clonotyping System-C57BL/6-AP kit is designed for the isotyping of C57BL/6 mouse monoclonal antibodies. It contains 2.5 mg of capture antibody and 1.0 mL of AP conjugated anti-mouse Ig, mouse IgA, mouse IgG1, mouse IgG2b, mouse IgG2c, mouse IgG3, mouse IgM, mouse κ, mouse λ, and pNPP substrate. The kit may also be utilized for quantitative studies of mouse immunoglobulins in samples such as serum, supernatant, and ascites when used in conjunction with the C57BL/6 Mouse Immunoglobulin Panel (SB Cat. No. 5300-01B).
Kit Components Goat Anti-Mouse Ig, Human ads-UNLB - 2.5 mg in 1.0 mL
Goat Anti-Mouse Ig, Human ads-AP - 1.0 mL
Goat Anti-Mouse IgA-AP - 1.0 mL
Goat Anti-Mouse IgG1, Human ads-AP - 1.0 mL
Goat Anti-Mouse IgG2b, Human ads-AP - 1.0 mL
Goat Anti-Mouse IgG2c, Human ads-AP - 1.0 mL
Goat Anti-Mouse IgG3, Human ads-AP - 1.0 mL
Goat Anti-Mouse IgM, Human ads-AP - 1.0 mL
Goat Anti-Mouse Kappa-AP - 1.0 mL
Goat Anti-Mouse Lambda-AP - 1.0 mL
pNPP Substrate Tablets - 20 x 5 mg
Format/Conjugate AP (Alkaline Phosphatase)
Buffer Formulation UNLB antibody - Borate buffered saline, pH 8.2 AP conjugates - 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications ELISA – Quality tested 1-4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796079
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References
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2. Hwang I, Park C, Luong T, Harrison KA, Birnbaumer L, Kehrl JH. The loss of Gnai2 and Gnai3 in B cells eliminates B lymphocyte compartments and leads to a hyper-IgM like syndrome. PLoS One. 2013;8(8):e72596. (ELISA - Serum)
3. Park KS, Bayles I, Szlachta-McGinn A, Paul J, Boiko J, Santos P, et al. Transcription elongation factor ELL2 drives Ig secretory-specific mRNA production and the unfolded protein response. J Immunol. 2014;193:4663-74. (ELISA - Serum)
4. Hwang I, Park C, Harrison K, Boularan C, Galés C, Kehrl JH. An essential role for RGS protein/Gαi2 interactions in B lymphocyte-directed cell migration and trafficking. J Immunol. 2015;194:2128-39. (ELISA - Serum)