Streptavidin-HRP

Cat. No.
7100-05  
Size
1.0 mL  
Price (USD)
$110.00 
Source Streptomyces avidinii
Specificity Biotin
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications ELISA – Quality tested 1-7
FLISA – Quality tested
Flow Cytometry – Quality tested 1,2,4,8-15
ELISpot – Reported in literature 4,6-8
Immunohistochemistry-Frozen Sections – Reported in literature 9,10,13,16-19
Immunohistochemistry-Paraffin Sections – Reported in literature 16,18,21
Immunocytochemistry – Reported in literature 11,17,19
Western Blot – Reported in literature 3,5,20
Multiplex – Reported in literature 22
Microarray – Reported in literature 23
Blocking – Reported in literature 24
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Paraffin embedded human gastric cancer tissue was stained with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
  • Paraffin embedded human colon cancer tissue was stained with Mouse Anti-Human MMP-9-UNLB (SB Cat. No. 12025-01) followed by Goat Anti-Mouse IgG2a, Human ads-BIOT (SB Cat. No. 1080-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
  • Paraffin embedded mouse adductor muscle day 7 post femoral artery ligation was stained with anti-Mac-3 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08) and then Streptavidin-HRP (SB Cat. No. 7100-05), hematoxylin, and AEC.

    Image from van den Borne P, Rygiel TP, Hoogendoorn A, Westerlaken GH, Boon L, Quax PH, et al. The CD200-CD200 receptor inhibitory axis controls arteriogenesis and local T lymphocyte influx. PLoS One. 2014;9(6):e98820. Figure 2(f)
  • Urine and serum samples were resolved by electrophoresis, transferred to PVDF membrane, and probed with biotinylated anti-human fH followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
    Lane 1 - Purified fH
    Lane 2 - Urine from IgAN patient
    Lane 3 - Serum from IgAN patient
    Lane 4 - Urine from healthy patient

    Image from Onda K, Ohsawa I, Ohi H, Tamano M, Mano S, Wakabayashi M, et al. Excretion of complement proteins and its activation marker C5b-9 in IgA nephropathy in relation to renal function. BMC Nephrol. 2011;12:64. Figure 4
  • ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG2bk. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7100-05).

Paraffin embedded human gastric cancer tissue was stained with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
 

Paraffin embedded human colon cancer tissue was stained with Mouse Anti-Human MMP-9-UNLB (SB Cat. No. 12025-01) followed by Goat Anti-Mouse IgG2a, Human ads-BIOT (SB Cat. No. 1080-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
 

Paraffin embedded mouse adductor muscle day 7 post femoral artery ligation was stained with anti-Mac-3 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08) and then Streptavidin-HRP (SB Cat. No. 7100-05), hematoxylin, and AEC.

Image from van den Borne P, Rygiel TP, Hoogendoorn A, Westerlaken GH, Boon L, Quax PH, et al. The CD200-CD200 receptor inhibitory axis controls arteriogenesis and local T lymphocyte influx. PLoS One. 2014;9(6):e98820. Figure 2(f)
 

Urine and serum samples were resolved by electrophoresis, transferred to PVDF membrane, and probed with biotinylated anti-human fH followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
Lane 1 - Purified fH
Lane 2 - Urine from IgAN patient
Lane 3 - Serum from IgAN patient
Lane 4 - Urine from healthy patient

Image from Onda K, Ohsawa I, Ohi H, Tamano M, Mano S, Wakabayashi M, et al. Excretion of complement proteins and its activation marker C5b-9 in IgA nephropathy in relation to renal function. BMC Nephrol. 2011;12:64. Figure 4
 

ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG2bk. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
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11. Moura IC, Arcos-Fajardo M, Sadaka C, Leroy V, Benhamou M, Novak J, et al. Glycosylation and size of IgA1 are essential for interaction with mesangial transferrin receptor in IgA nephropathy. J Am Soc Nephrol. 2004;15:622-34. (FC, ICC)
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