Goat F(ab')2 Anti-Human Kappa, Mouse ads-FITC

Cat. No.
2063-02  
Size
0.5 mg  
Price (USD)
$260.00 
Specificity Reacts with human κ light chains
Source Pepsin digest of Goat Anti-Human Kappa, Mouse ads (SB Cat. No. 2061)
Cross Adsorption Human λ light chains; mouse immunoglobulins and pooled sera; may react with κ light chains from other species
Isotype Goat F(ab')2 IgG
Isotype Control Goat F(ab')2 IgG-FITC
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 3
FLISA
Flow Cytometry 1,2
Other referenced applications for relevant formats include -
ELISpot 3
Stimulation 3,4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795745
  • FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Human Kappa, Mouse ads-FITC (SB Cat. No. 2063-02).

FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Human Kappa, Mouse ads-FITC (SB Cat. No. 2063-02).
 
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References
1. Akamatsu Y, Pakabunto K, Xu Z, Zhang Y, Tsurushita N. Whole IgG surface display on mammalian cells: Application to isolation of neutralizing chicken monoclonal anti-IL-12 antibodies. J Immunol Methods. 2007;327:40-52. (FC)
2. Wilson TJ, Fuchs A, Colonna M. Cutting edge: human FcRL4 and FcRL5 are receptors for IgA and IgG. J Immunol. 2012;188:4741-5. (FC)
3. Velez M, Kane M, Liu SM, Gauld SB, Cambier JC, Torres RM, et al. Ig allotypic inclusion does not prevent B cell development or response. J Immunol. 2007;179:1049-57. (ELISA, ELISPOT, Stim)
4. Hoogeboom R, van Kessel KP, Hochstenbach F, Wormhoudt TA, Reinten RJ, Wagner K, et al. A mutated B cell chronic lymphocytic leukemia subset that recognizes and responds to fungi. J Exp Med. 2013;210:59-70. (Stim)