Goat Anti-Rabbit Ig, Human ads-PE

Cat. No.
4010-09  
Size
0.5 mg  
Price (USD)
$320.00 
Specificity Reacts with the heavy and light chains of rabbit IgG and IgM
Source Pooled antisera from goats hyperimmunized with rabbit IgG
Cross Adsorption Human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on pooled rabbit IgG covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-PE
Format/Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-3
FLISA
Flow Cytometry 4
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 5-7
Immunohistochemistry-Paraffin Sections 3,8
Immunocytochemistry 9-14
Western Blot 15-25
Immunoprecipitation 26
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795920
  • Lysates from mouse embryonic fibroblasts expressing no Bak (Bax-/-Bak-/- (DKO)), mouse Bak (Bax-/-), or WT human Bak (in DKO) were resolved by electrophoresis, transferred to nitrocellulose membrane, and probed with anti-Bak followed by Goat Anti-Rabbit Ig, Human ads-HRP (SB Cat. No. 4010-05) and chemiluminescent detection.

    Image from Alsop AE, Fennell SC, Bartolo RC, Tan IK, Dewson G, Kluck RM. Dissociation of Bak α1 helix from the core and latch domains is required for apoptosis. Nat Commun. 2015;6:6841. Figure 1(a)

Lysates from mouse embryonic fibroblasts expressing no Bak (Bax-/-Bak-/- (DKO)), mouse Bak (Bax-/-), or WT human Bak (in DKO) were resolved by electrophoresis, transferred to nitrocellulose membrane, and probed with anti-Bak followed by Goat Anti-Rabbit Ig, Human ads-HRP (SB Cat. No. 4010-05) and chemiluminescent detection.

Image from Alsop AE, Fennell SC, Bartolo RC, Tan IK, Dewson G, Kluck RM. Dissociation of Bak α1 helix from the core and latch domains is required for apoptosis. Nat Commun. 2015;6:6841. Figure 1(a)
 
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