Rat Anti-Mouse LPAM-1-UNLB

Cat. No.
1870-01  
Size
0.5 mg  
Price (USD)
$218.00 
Clone DATK32
Isotype Rat (Fischer) IgG2aκ
Isotype Control Rat IgG2a-UNLB
Immunogen AKR/Cum mouse T lymphoma cell line TK-1
Specificity Mouse LPAM-1
Alternalte Name(s) Integrin α4β7, lymphocyte Peyer's patch adhesion molecule
Description The lymphocyte Peyer’s patch adhesion molecule (LPAM-1), also known as integrin α4β7, is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes but is also present on NK cells and eosinophils. The α4β7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM-1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer’s patches and intestinal lamina propria. The monoclonal antibody DATK32 recognizes a conformational epitope on the heterodimer.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,3-5
Immunohistochemistry-Frozen Sections – Reported in literature 4
Blocking – Reported in literature 1-3
Immunoprecipitation – Reported in literature 4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795417
Gene ID
16401 (Mouse)
16421 (Mouse)
Gene Symbol
Itga4 (Mouse)
Itgb7 (Mouse)
Gene Aliases CD49D; Itga4B; Ly69
Uniprot ID
Q00651 (Mouse)
P26011 (Mouse)
Uniprot Name
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse LPAM-1-UNLB (SB Cat. No. 1870-01) followed by Mouse Anti-Rat IgG2a-CY5 (SB Cat. No. 3065-15).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse LPAM-1-UNLB (SB Cat. No. 1870-01) followed by Mouse Anti-Rat IgG2a-CY5 (SB Cat. No. 3065-15).
 
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References
1. Andrew DP, Berlin C, Honda S, Yoshino T, Hamann A, Holzmann B, et al. Distinct but overlapping epitopes are involved in α4β7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol. 1994;153:3847-61. (Immunogen, FC, Block)
2. Berlin C, Berg EL, Briskin MJ, Andrew DP, Kilshaw PJ, Holzmann B, et al. α4β7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell. 1993;74:185-95. (Block)
3. Katayama Y, Hidalgo A, Peired A, Frenette PS. Integrin α4β7 and its counterreceptor MAdCAM-1 contribute to hematopoietic progenitor recruitment into bone marrow following transplantation. Blood. 2004;104:2020-26. (Block, FC)
4. Bogetto L, Gabriele E, Cariati R, Dolcetti R, Spressotto P, Doglioni C, et al. Bidirectional induction of the cognate receptor-ligand α4-VCAM-1 pair defines a novel mechanism of tumor intravasation. Blood. 2000;95:2397-406. (FC, IP, IHC-FS)
5. Seo G, Jang Y, Kim H, Lee M, Park M, Park S, et al. Retinoic acid, acting as a highly specific IgA isotype switch factor, cooperates with TGF-β1 to enhance the overall IgA response. J Leukoc Biol. 2013;94:325-35. (FC)