The SouthernBiotech Mycoplasma Detection Kit is designed to specifically detect potential Mycoplasma contamination in cell cultures. The kit incorporates polymerase chain reaction (PCR) to amplify the conserved 16S ribosomal RNA coding region within the Mycoplasma genome, thereby providing an extensive, highly sensitive, and efficient detection method.
Carefully determined primer sequences cover three genera of Mycoplasmatales (Mycoplasma, Acholeplasma, and Ureaplasma) which allows the kit to detect over 95% of potential cell culture infections. The PCR technology in our Mycoplasma Detection Kit is easy to use and rapid, with results typically obtained in less than 3 hours. The kit is also highly sensitive and can detect as little as 2-5 femtograms of Mycoplasma DNA in 100 μL of test sample supernatant.