SouthernBiotech anti-human secondary antibodies are extensively characterized, affinity-purified polyclonal or monoclonal antibodies with specificities to the human immunoglobulin heavy chain classes and subclasses, light chains, and fragments. We offer anti-human secondary antibodies produced in goat, rabbit, and mouse. They are valuable in the detection of chimeric, humanized, and fully human primary antibodies and are available in a variety of formats including unconjugated as well as enzyme, biotinylated, and fluorescent conjugates for assays such as ELISA, western blot, flow cytometry, multiplex, and immunohistochemistry.
Since monoclonal antibodies will bind a single epitope on the antibody target, they are highly specific, deliver superior lot-to-lot consistency, and are often well characterized in the literature. Our polyclonal anti-human secondary antibodies can provide improved sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Selected antibodies have been cross-adsorbed against IgG and/or serum proteins from other species to generate antibodies with minimal cross-reactivity to those species. It is important to use a secondary antibody that has minimal cross-reactivity to the species of your sample. With a comprehensive offering of polyclonal and monoclonal anti-human class and subclass specific secondary antibodies (e.g., IgG1, IgG4, IgA2, IgM, IgD), multiple analytes can be detected simultaneously in an assay. We have a broad selection of whole molecule and F(ab’)2 fragment polyclonal and monoclonal anti-human antibodies, all produced and validated in-house, for use in your specific assay.