Primary Antibodies

With a wide range of high-quality primary antibodies made in-house by our dedicated team, you’re sure to find a reliable reagent to drive your success.

What are primary antibodies?

Primary antibodies recognize and bind target antigens such as cell surface markers, signaling molecules, and post-translationally modified proteins with high specificity and affinity. This makes them useful for detecting and isolating various biomolecules with techniques including western blot, ELISA, immunoprecipitation, and flow cytometry.

Depending on how they are produced, primary antibodies are classified as either polyclonal or monoclonal. The main difference is that polyclonal antibodies recognize multiple epitopes, while monoclonal antibodies recognize just a single epitope. Both polyclonals and monoclonals can be labeled to allow for direct detection or can alternatively be paired with a suitable secondary antibody for indirect detection.

Primary antibodies are distinct from secondary antibodies, which recognize antibodies from a particular host species and of a specific isotype. Secondary antibodies are generally polyclonal and are named according to the host species and target species. For example, goat anti-mouse IgG1 secondary antibodies are raised in a goat and recognize mouse antibodies of the IgG1 isotype.

Shop popular primary antibodies by type

Types of primary antibodies

  • Monoclonal Antibodies
    Monoclonal antibodies are produced using hybridoma technology or recombinant methods. Their advantages include high specificity, consistent batch-to-batch performance, and stable long-term supply.
  • Conjugated Antibodies
    Conjugated antibodies are widely used for multiplexed protein detection with techniques such as ELISA, immunohistochemistry (IHC) and flow cytometry. Available labels include enzymes, fluorescent dyes, and biotin.
  • Veterinary Antibodies
    Antibodies targeting animal-specific proteins represent essential tools for veterinary research, as well as for interrogating human disease models. Common research applications include ELISA, IHC, and various functional assays.
  • Epitope Tag Antibodies
    Epitope tags are added to the N- or C- termini of proteins to facilitate their detection or isolation. They include short peptide sequences and larger protein molecules and are especially useful for studying targets for which specific antibodies are not available.

Shop popular primary antibodies by research area

Primary antibodies by research area

  • Immunology Antibodies
    Immunology antibodies are used for characterizing the various cell types and signaling molecules involved in protecting against disease. They target cell surface markers, cytokines, and other biomolecules using techniques such as flow cytometry, ELISA, and IHC.
  • Cytoskeletal and ECM Antibodies
    The cytoskeleton and ECM perform many essential functions, including roles in cellular proliferation, differentiation, and migration. Antibodies targeting proteins such as actin, tubulin, and collagen allow for studying the cytoskeleton and ECM under conditions of health and disease.
  • Apoptosis Antibodies
    Apoptosis is a type of programmed cell death that is often dysregulated in pathologic conditions. Common antibody-based assays for studying apoptosis include methods for measuring caspase activation, cytochrome C release, and glutathione depletion.
  • Signal Transduction Antibodies
    Signal transduction antibodies are used for studying cellular responses to signaling molecules such as hormones, neurotransmitters, and growth factors. Aberrant signal transduction is linked to conditions including cancer, arthritis, and neurodegeneration.

Choose SouthernBiotech for success

We recognize that you have many choices when it comes to buying primary antibodies, by partnering with SouthernBiotech, you can count on:

  • Quality proven over four decades of success. Scientists have been buying antibodies from us since our inception in 1982, long before many of our competitors were even founded.
  • Fast delivery and supply chain security thanks to our all in-house production. Whatever product or service you purchase from us, you can depend on the timely delivery of a high-quality product because all production is in our hands in our own facility.
  • Superior customer support. Our highly-skilled team is invested in your success and will work closely with you to ensure our products work the way you need them to.

We ensure reliability and specificity

The success of any immunoassay hinges on the quality of the antibody reagents that are used. At SouthernBiotech, we produce all of our antibodies in-house and validate them with techniques including:

  • Flow cytometry
  • Western blot
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunoprecipitation

By maintaining complete control over our antibody production workflow, we provide competitive pricing and next-day delivery, backed by fast and comprehensive technical support, giving you total confidence in your experimental results.

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