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Apoptosis Kits & Reagents

ApoScreen® Annexin V Apoptosis Kits employ a fluorescein-conjugated Annexin V in concert with propidium iodide (PI) or 7-aminoactinomycin D (7-AAD) to evaluate subpopulations of cells undergoing apoptosis. During the early stages of apoptosis, cells begin to display phosphatidylserine (PS) on the outer cell membrane where it is readily detectable by staining the cells with Annexin V. As the plasma membrane becomes increasingly permeable during the later stages of apoptosis, PI and 7-AAD can readily move across the cell membrane and bind to cellular DNA, providing a means for identifying those cells that have lost membrane integrity through mechanisms including necrosis.

Analysis may be performed by flow cytometry or examined with a fluorescence microscope. Apoptosis screening reagents are also available as standalone reagents for the generation of assays for your specific needs.