Goat Anti-Human Kappa, Mouse ads-Alexa Fluor® 647

Cat. No.
2061-31  
Size
1.0 mg  
Price (USD)
$375.00 
Specificity Reacts with human κ light chains
Source Pooled antisera from goats hyperimmunized with human κ light chains
Cross Adsorption Human λ light chains; mouse immunoglobulins and pooled sera; may react with κ light chains from other species
Purification Affinity chromatography on human κ light chains covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-Alexa Fluor® 647
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1
FLISA
Flow Cytometry
Recommended Dilutions Please refer to product specific Technical Bulletin
Gene ID
3514 (Human)
Gene Symbol
IGKC (Human)
Gene Aliases HCAK1D; Km; IGKC
Uniprot ID
P01834 (Human)
Uniprot Name
  • FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa, Mouse ads-AF647 (SB Cat. No. 2061-31).
  • CD19+ human B-lymphocytes were stained with Goat Anti-Human Kappa, Mouse ads-AF647 (SB Cat. 2061-31) and Goat F(ab’)2 Anti-Human Lambda, Mouse ads-FITC (SB Cat. No. 2073-02).

FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa, Mouse ads-AF647 (SB Cat. No. 2061-31).
 

CD19+ human B-lymphocytes were stained with Goat Anti-Human Kappa, Mouse ads-AF647 (SB Cat. 2061-31) and Goat F(ab’)2 Anti-Human Lambda, Mouse ads-FITC (SB Cat. No. 2073-02).
 
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References
1. Maurer MF, Garrigues U, Jaspers SR, Meengs B, Rixon MW, Stevens BL, et al. Generation and characterization of human anti-human IL-21 neutralizing monoclonal antibodies. MABs. 2012;4:1:69-83. (ELISA)
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